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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Late blight-resistant Jalene potato
EN
Jal99
No
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Organization:National Root Crops Research Institute (NRCRI)Academic or research instituteKm 8 Umuahia Ikot Ekpene Road, P.M.B 7006, Umuahia Abia stateUmudike, Abia
, NigeriaPhone: +234 818 762 0664,Fax:Email: nrcriumudike@yahoo.com,Website:
The potato (Solanum tuberosum) was modified to express three late blight resistance genes. To achieve this, the potato expresses Solanum bulbocastanum Phytophthora infestans resistance gene blb1, S. bulbocastanum Phytophthora infestans resistance gene blb2 and Solanum venturii Phytophthora infestans resistance gene 1 (Rpi-vnt1.1), which recognize pathogen proteins to mediate a hypersensitive defense response. In addition to the resistance genes, the potato expresses Escherichia coli neomycin phosphotransferase, which was used for kanamycin selection during transformation.
Note: For the transformation event, a line has not be selected. Thus, a generic transformation event was chosen.
Note: For the transformation event, a line has not be selected. Thus, a generic transformation event was chosen.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
EN
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Late blight-resistant Desiree potato| National Root Crops Research Institute(NRCRI) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests, Selectable marker genes and reporter genes
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Late blight-resistant Victoria potato| National Root Crops Research Institute(NRCRI) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests, Selectable marker genes and reporter genes
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Late blight-resistant Tigoni potato| National Root Crops Research Institute(NRCRI) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests, Selectable marker genes and reporter genes
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Late blight-resistant Shangi potato| National Root Crops Research Institute(NRCRI) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests, Selectable marker genes and reporter genes
pCIP99
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- Agrobacterium-mediated DNA transfer
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0.026 kb
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0.204 kb
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0.798 kb
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0.781 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-109062-2 Phytophthora infestans Resistance gene 1 Promoter | Solanum venturii (Wild Potato, SOLVN)Promoter
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BCH-GENE-SCBD-102155-7 Phytophthora infestans Resistance gene 1 | Solanum venturii (Wild Potato, SOLVN)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-109063-2 Phytophthora infestans Resistance gene 1 terminator | Solanum venturii (Wild Potato, SOLVN)Terminator
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BCH-GENE-SCBD-103777-3 Phytophthora infestans Resistance gene 1 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Promoter
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BCH-GENE-SCBD-41317-6 Phytophthora infestans Resistance gene 1 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-103778-3 Phytophthora infestans Resistance gene 1 terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Terminator
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BCH-GENE-SCBD-103775-2 Phytophthora infestans Resistance gene 2 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Promoter
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BCH-GENE-SCBD-41318-4 Phytophthora infestans Resistance gene 2 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Protein coding sequence | Resistance to diseases and pests (Phytophthora infestans resistance)
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BCH-GENE-SCBD-103776-2 Phytophthora infestans Resistance gene 2 Terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Terminator
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
The modified potato (Solanum tuberosum) contains four gene cassettes: Escherichia coli neomycin phosphotransferase (nptII); Solanum venturii Phytophthora infestans resistance gene 1 (Rpi-vnt1.1); Solanum bulbocastanum Phytophthora infestans resistance gene blb1 (Rpi-blb1; RB) and S. bulbocastanum Phytophthora infestans resistance gene blb2 (Rpi-blb2)
The nptII coding sequence is under control of a Cauliflower mosaic virus 35S promoter and terminator.
The Rpi-blb1, Rpi-blb2 and Rpi-vnt1.1 coding sequences are under control of their native promoters and terminators.
Note:
The nptII coding sequence is under control of a Cauliflower mosaic virus 35S promoter and terminator.
The Rpi-blb1, Rpi-blb2 and Rpi-vnt1.1 coding sequences are under control of their native promoters and terminators.
Note:
- The genetic element sizes of the promoters and terminators of Rpi-blb1, Rpi-blb2 and Rpi-vnt1.1 are not clear.
EN
- Food
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
| Living Modified Organism | Related LMO(s) | 4 | |
