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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Violet chrysanthemum
EN
2022-01
Yes
SN-2Ø22Ø1-4
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Person:IFD802/454 Saint Kilda RoadMelbourne, Victoria
3004, AustraliaPhone: 61 3 94199844,Fax:Email: info@florigene.com,Website:Related OrganizationInternational Flower Developments Pty Ltd (IFD)Private sector (business and industry)802/454 Saint Kilda RoadMelbourne, Victoria
3004, AustraliaPhone: 61 3 94199844,Fax:Email: info@florigene.com,Website:
The chrysanthemum (Chrysanthemum x morifolium) was modified for violet flower coloration. The modified chrysanthemum contains Campanula medium flavonoid 3’,5’-hydroxylase and Clitoria ternatea UDP-glucose:anthocyanin 3’,5’-O-glucosyltransferase. Flavonoid 3’,5’-hydroxylase converts pink to red cyanidin-based precursor into purple to blue delphinidin-type pigments. UDP-glucose:anthocyanin 3’,5’-O-glucosyltransferase efficiently glucosylates both cyanidin-type and delphinidin-type pigments, which changes the purple pigment to have a blue colour. The modified chrysanthemums have slightly smaller flowers and are about 5% shorter compared to the non-modified lines.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-105667-3 Organism Chrysanthemum morifolium (Florists chrysanthemum, Mum, Florist's daisy, Hardy garden mum, CHRMO)Ornamentals
Cultivar Sei Shawl
EN
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NS-2Ø18Ø6-5 - Blue-violet chrysanthemum| International Flower Developments Pty Ltd(IFD) | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin), Selectable marker genes and reporter genes
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NS-2Ø8133-5 - Violet chrysanthemum| International Flower Developments Pty Ltd(IFD) | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin), Selectable marker genes and reporter genes
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SN-2Ø37Ø1-1 - Blue chrysanthemum| International Flower Developments Pty Ltd(IFD) | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin), Selectable marker genes and reporter genes
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NS-2128Ø1-2 - Blue-violet chrysanthemum| International Flower Developments Pty Ltd(IFD) | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin), Selectable marker genes and reporter genes
pB423
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-263134-2 Heat shock protein 18.2 terminator | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Terminator
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BCH-GENE-SCBD-263128-2 Flavanone 3-hydroxylase gene promoter | Chrysanthemum morifolium (Florists chrysanthemum, Mum, Florist's daisy, Hardy garden mum, CHRMO)Promoter
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-110414-1 Alcohol dehydrogenase 5′UTR | Nicotiana tabacum (Tobacco, TOBAC )Enhancer
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BCH-GENE-SCBD-109829-1 Flavonoid 3', 5' hydroxylase gene | Campanula medium (Canterbury bells, Cup-and-saucer, Bellflower, CAMME)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-263133-3 UDP-glucose:anthocyanin 3’,5’-O-glucosyltransferase | Clitoria ternatea (Bluebellvine, Asian pigeonwings, Butterfly pea, Blue pea, CLITE)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
The modified chrysanthemums contain three gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII), Campanula medium flavonoid 3’,5’-hydroxylase (f3’5’h) and Clitoria ternatea UDP-glucose:anthocyanin 3’,5’-O-glucosyltransferase (ua3’5’gt).
The nptII coding sequence is under control of an Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator. The promoter in this cassette is constitutive and is expected to be active in all plant tissues.
The f3’5’h coding sequence is under control of chrysanthemum flavanone 3-hydroxylase (f3h) promoter and Arabidopsis thaliana heat shock protein 18.2 terminator. The promoter is petal-specific and thus expression of F3’5’H is expected to be restricted to the petals. A Nicotiana tabacum alcohol dehydrogenase 5’ untranslated region intron was included as a translational enhancer. The terminator is also expected to enhance gene expression.
The ua3’5’gt coding sequence is under control of a f3h promoter and nos terminator. A N. tabacum alcohol dehydrogenase 5’ untranslated region intron was included as a translational enhancer. Due to the nature of the petal-specific nature of the promoter, expression of UA3’5’GT is expected to be restricted to the petals.
Southern blot analysis indicated that the modified chrysanthemums contained one insert.
Southern blot analysis indicated that the modified chrysanthemums contained one insert.
EN
- Ornamental
EN
EN
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