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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant, herbicide-tolerant maize
EN
4114 × MON89034
Yes
DP-ØØ4114-3 × MON-89Ø34-3
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Organization:Pioneer Hi-Bred International Inc. ()Private sector (business and industry)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, United States of AmericaPhone: +1 515 535-3200,Fax:Email:Website: www.pioneer.com/, -
Organization:Bayer CropScience ()Phone:Fax:Email:Website: http://www.bayercropscience.com,
The maize (Zea mays) was produced through crossing modified parental lines for resistance to insect pests and tolerance to herbicides. For protection from Lepidoptera insects, the maize expresses Bacillus thuringiensis Cry1A.105, Cry1F and Cry2Ab2 proteins, which have a pore-forming mode of action that selectively damages the midgut epithelium line of feeding larvae and leads to death. For protection from Coleoptera insects, the maize expresses B. thuringiensis Cry34Ab1 and Cry35Ab1, which also has a pore-forming mode of action in the midgut epithelium lining. For tolerance to glufosinate, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase gene, which inactivates the herbicidal compound through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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BCH-LMO-SCBD-43773-18 Living Modified Organism MON-89Ø34-3 - YieldGard™ VT Pro™Monsanto Company | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-LMO-SCBD-104976-2 Living Modified Organism DP-ØØ4114-3 - Insect-Resistant and Herbicide-Tolerant MaizePioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Coleoptera (beetles), Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
EN
PHP27118; PV-ZMIR245
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100354-6 5' untranslated leader from chlorophyll a/b-binding protein | Triticum aestivum (Wheat)Leader sequence
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-43771-9 Cry1A.105 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100356-6 Heat shock protein 17.3 terminator | Triticum aestivum (Wheat)Terminator
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100360-4 Transit peptide and first intron of Rubisco SSU | Zea mays (Maize, Corn, MAIZE)Transit signal
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BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-14994-9 Cry34Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
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BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | Solanum tuberosum (Potato, SOLTU)Terminator
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BCH-GENE-SCBD-100368-6 Peroxidase gene promoter | Triticum aestivum (Wheat)Promoter
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BCH-GENE-SCBD-14995-8 Cry35Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
DNA insert from MON89034 (MON-89Ø34-3) vector PV-ZMIR245:
Maize line MON89034 expresses two Bt-toxins encoded by Bacillus thuringiensis cry1A.105 and cry2Ab2.
Transcription of cry1A.105 begins at the Cauliflower mosaic virus (CaMV) Enhanced 35S promoter and finishes at the wheat (Triticum aestivum) wheat heat shock protein 17.3 terminator. The transcript initially includes (5' to 3'): wheat 5' untranslated leader from the chlorophyll a/b-binding protein, Oryza sativa (rice) actin 1 intron and cry1A.105. The wheat 5' untranslated leader sequence and the rice intron enhance the expression of cry1A.105.
Transcription of cry2Ab2 commences from the Figwort mosaic virus (FMV) 34S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript initially includes (5' to 3'): maize heat shock protein 70 (hsp70) intron, maize transit peptide and first intron from the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and cry2Ab32. The hsp70 regulates and enhances gene expression, while the transit peptide targets Cry2Ab2 to the chloroplast.
Note:
Maize line MON89034 expresses two Bt-toxins encoded by Bacillus thuringiensis cry1A.105 and cry2Ab2.
Transcription of cry1A.105 begins at the Cauliflower mosaic virus (CaMV) Enhanced 35S promoter and finishes at the wheat (Triticum aestivum) wheat heat shock protein 17.3 terminator. The transcript initially includes (5' to 3'): wheat 5' untranslated leader from the chlorophyll a/b-binding protein, Oryza sativa (rice) actin 1 intron and cry1A.105. The wheat 5' untranslated leader sequence and the rice intron enhance the expression of cry1A.105.
Transcription of cry2Ab2 commences from the Figwort mosaic virus (FMV) 34S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript initially includes (5' to 3'): maize heat shock protein 70 (hsp70) intron, maize transit peptide and first intron from the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and cry2Ab32. The hsp70 regulates and enhances gene expression, while the transit peptide targets Cry2Ab2 to the chloroplast.
Note:
- The viral promoters are expected to be constitutively active and promote high levels of transcription.
- The coding sequence of cry2Ab2 was codon-optimized for expression within plant systems.
- A second T-DNA insertion (containing CaMV 35S promoter, Escherichia coli neomycin phosphotransferase and A. tumefaciens nos terminator) was initially inserted into the genome for kanamycin selection during transformation. However, once transformants were regenerated, the selectable marker was bred out of the parental line using convention breeding techniques.
- Southern blot analyses indicated a single copy of the cry1A.105 and the cry2Ab2 cassettes. No backbone plasmid DNA or nptII sequences were detected. PCR and DNA sequence analyses provided the complete DNA sequence of the insert and confirmed the organization of the elements within the insert. Furthermore, sequence analysis indicated that MON89034 no longer has the duplicated enhancer elements compared to the original e35S promoter in PV-ZMIR245, possibly due to a recombination event that resulted in its deletion.
DNA insert from 4114 (DP-ØØ4114-3) vector PHP27118
The DNA insert contains four gene cassettes: Bacillus thuringiensis cry1F, B. thuringiensis cry34Ab1, B. thuringiensis cry35Ab1 and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).
Transcription of the cry1F is under control of a Zea mays ubiquitin promoter and an Agrobacterium tumefaciens ORF25 terminator. A Z. mays ubiquitin intron 1 sequence is also present to enhance the expression of cry1F.
Transcription of the cry34Ab1 is under control of a Z. mays ubiquitin promoter and Solanum tuberosum proteinase inhibitor II terminator. A Z. mays ubiquitin intron 1 sequence is also present to enhance the expression of cry34Ab1.
Transcription of cry35Ab1 is under control of Triticum aestivum peroxidase promoter and S. tuberosum proteinase inhibitor II terminator.
Transcription of pat is under control of Cauliflower mosaic virus (CaMV) 35S promoter and CaMV 35S terminator.
Note:
Transcription of the cry34Ab1 is under control of a Z. mays ubiquitin promoter and Solanum tuberosum proteinase inhibitor II terminator. A Z. mays ubiquitin intron 1 sequence is also present to enhance the expression of cry34Ab1.
Transcription of cry35Ab1 is under control of Triticum aestivum peroxidase promoter and S. tuberosum proteinase inhibitor II terminator.
Transcription of pat is under control of Cauliflower mosaic virus (CaMV) 35S promoter and CaMV 35S terminator.
Note:
- The ubiquitin gene promoter element is made up of the promoter region (900bp) and the 5' untranslated region (83bp) of the ubiquitin gene.
- Transcription is expected to occur at elevated levels for the present gene cassettes.
- Southern blot analyses indicated that a single, intact transformation cassette was inserted into the genome of 4114 maize with no integration of the backbone of plasmid PHP27118.
For more information, kindly refer to the parental LMO records.
EN
- Feed
- Food
- DP-ØØ4114-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-89Ø34-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-89Ø34-3 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
EN
- EUginius - DP4114 x MON89034 [ English ]
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