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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Bollgard®, Roundup Ready® and LibertyLink® Cotton
EN
MON531 × MON1445 × LLCotton25
Yes
MON-Ø531-6 x MON-Ø1445-2 x ACS-GHØØ1-3
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Organization:GENSUS S.A ()Private sector (business and industry)San Vladimiro 3056, San Isidro (B1642GMB) Buenos Aires, ArgentinaSan Isidro, Buenos Aires
B1642GMB,Phone:Fax:Email: consultas@gensus.com.ar,Website: https://gensus.com.ar/,
The cotton (Gossypium hirsutum) was produced through cross breeding of modified parental varieties MON531 × MON1445 and ACS-GHØØ1-3. For resistance to lepidopteran insect pests, the cotton expresses Bacillus thuringiensis Cry1Ac, an insecticidal crystal protein active against susceptible Lepidoptera including cotton bollworm, pink bollworm and tobacco budworm. The protein acts through disruption of the insect midgut following ingestion. For tolerance to glyphosate, the cotton expresses the CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS), which has reduced sensitivity to glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway. For tolerance to glufosinate ammonium herbicides, the cotton expresses phosphinothricin N-acetyltransferase (PAT), derived from Streptomyces hygroscopicus, which inactivates glufosinate through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-LMO-SCBD-14775-17 Living Modified Organism MON-ØØ531-6 - Bollgard™ cottonMonsanto | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-LMO-SCBD-14851-8 Living Modified Organism ACS-GHØØ1-3 - Liberty Link™ cottonBayer CropScience | Resistance to herbicides (Glufosinate)
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BCH-LMO-SCBD-14880-14 Living Modified Organism MON-Ø1445-2 - Roundup Ready™ cottonMonsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to herbicides (Glyphosate)
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BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)Crops
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MON-ØØ531-6 × MON-Ø1445-2 - Roundup Ready™ Bollgard™ Cotton| Monsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glyphosate)
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ACS-GHØØ1-3 × MON-15985-7 - Liberty Link™ Bollgard II™ cotton| Bayer CropScience | Resistance to antibiotics (Streptomycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
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BCS-GHØØ2-5 × ACS-GHØØ1-3 - GlyTol™ Liberty Link™ cotton| Resistance to herbicides (Glufosinate, Glyphosate)
PV-GHBK04, pGSV71 and PV-GHGT07
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- Agrobacterium-mediated DNA transfer
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)Terminator
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Streptomycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
DNA insert from MON-ØØ531-6
The transforming plasmid PV-GHBK04 contained two gene cassettes expressing Cry1Ac and NPTII.
-The cry1Ac coding sequence is under control of Cauliflower mosaic virus (CaMV) 35S enhanced promoter and Glycine max α' subunit of β-conglycinin terminator. See notes below about the partial cry1Ac cassette and antibiotic resistance gene aad.
-The nptII coding sequence is under control of a CaMV 35S promoter and and Agrobacterium tumefaciens nopaline synthase terminator.
Note:
-The cry1Ac coding sequence is under control of Cauliflower mosaic virus (CaMV) 35S enhanced promoter and Glycine max α' subunit of β-conglycinin terminator. See notes below about the partial cry1Ac cassette and antibiotic resistance gene aad.
-The nptII coding sequence is under control of a CaMV 35S promoter and and Agrobacterium tumefaciens nopaline synthase terminator.
Note:
- Due to the constitutive nature of the viral promoters, high levels of expression are expected in all plant tissues.
- The cry1Ac coding sequence was codon-optimized for expression in plants. The codon optimization resulted in a single amino acid change (leucine substituted for serine) at position 766 (L766S). The sequence was originally sourced from B. thuringiensis subsp. kurstaki HD-73.
- Southern blot analysis indicated that the LMO contains a single active copy and a partial inactive copy of the cry1Ac gene both of which are linked.
- The plasmid also contains the antibiotic resistance 3"(9)-O-aminoglycoside adenylyltransferase (aad) gene. This gene confers resistance to the antibiotics spectinomycin and streptomycin, and facilitated the selection of bacteria containing the plasmid in the initial steps of transforming the cotton tissue. The aad gene is under the control of a bacterial promoter. Studies using ELISA testing have indicated that there there is no detectable expression of the aad gene in the modified line.
DNA insert from MON-Ø1445-2
The transforming plasmid PV-GHGT07 contained gene cassettes expressing CP4 EPSPS and NPTII.
-The cp4 epsps coding sequence is under control of the Modified Figwort Virus 35S promoter and the Pisum sativum rbcS E9 terminator. The coding sequence includes the Arabidopsis thaliana EPSPS chloroplast transit peptide (CTP2), which directs the expressed protein to the chloroplast.
-The cp4 epsps coding sequence is under control of the Modified Figwort Virus 35S promoter and the Pisum sativum rbcS E9 terminator. The coding sequence includes the Arabidopsis thaliana EPSPS chloroplast transit peptide (CTP2), which directs the expressed protein to the chloroplast.
-The nptII coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase terminator.
Note:
Due to the constitutive nature of the viral promoters, expression is expected in most plant tissues.
- The CP4 EPSPS protein is targeted to the chloroplast through the Arabidopsis thaliana EPSPS chloroplast transit peptide (CTP2). Following transport into the chloroplast, the transit peptide is cleaved, producing the mature CP4 EPSPS protein.
- Southern blot analysis indicated that the modified line contains a single insertion event comprising approximately 6.1 kb of DNA. Molecular characterization demonstrated that the integrated DNA includes the cp4 epsps, nptII and aad sequences, together with a truncated oriV sequence.
- The gox gene cassette originally present in the transformation plasmid PV-GHGT07 was not integrated into the cotton genome.
- The plasmid also contains the antibiotic resistance 3''(9)-O-aminoglycoside adenylyltransferase (aad) gene. This gene confers resistance to the antibiotics spectinomycin and streptomycin and facilitated the selection of bacteria containing the plasmid during vector construction. The aad gene is under the control of a bacterial promoter and is not expected to be expressed in plant tissues. No detectable expression of the AAD protein was observed in the modified line.
DNA insert from ACS-GHØØ1-3
The transforming plasmid pGSV71 contained one gene cassettes expressing the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar). The bar coding sequence is under control of a Cauliflower mosaic virus 35S promoter and an Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the viral promoter, high levels of transcription are expected from this gene cassette.
Note:
Note:
- The initial two codons of the N-terminal of the bar coding sequence were synthetically modified to plant-preferred codons.
- Southern blot analysis indicated that a single intact copy of the T-DNA was integrated into the cotton genome and no vector backbone sequences were present.
EN
- Feed
- Fiber/textile
- Food
The presence of each individual event can be detected by PCR using oligonucleotide sequences specific to each event. In this case, the method is based on detecting the simultaneous presence of each individual event from DNA extracted from a single biological sample.
EN
- Argentina Resolución 132/2025 [ Spanish ]
- ISAAA database - MON-ØØ531-6 x MON-Ø1445-2 x ACS-GHØØ1-3 [ English ]
- OECD Biotrack database - MON-ØØ531-6xMON-Ø1445-2xACS-GHØØ1-3 [ English ]
- EUginius - MON531 [ English ]
- EUginius - MON1445 [ English ]
- EUginius - LLcotton25 [ English ]
