BPS-A1Ø2Ø-5 - Amadea potato | BCH-LMO-SCBD-48075 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 29 Apr 2020
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Amadea potato
EN
AM04-1020
Yes
BPS-A1Ø2Ø-5
The potato was modified for increased amylopectin and decreased amylose content. To achieve the reduction in amylose, an RNA interference cassette was introduced into the genome. The cassette encodes inverted repeats of a portion of granule bound starch synthase (gbss). The formation of hairpin RNA (hpRNA; double-stranded structure) triggers an RNAi response that silences the expression of the endogenous gbss through targetted degradation of the transcript and thus preventing the production of the GBSS protein. The reduction in enzyme activity resulted in a 98% increase in amylopectin content in the modified potato. Additionally, the insertion contains an Arabidopsis thaliana acetohydroxyacid synthase selectable marker for imidazolinone herbicide selection during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Potato cultivar Kuras
EN
Characteristics of the modification process
Derivate of pPZP200
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | altered carbohydrate composition: increased amylopectin content
  • BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-115566-1 Granule bound starch synthase spacer | Solanum tuberosum (Potato, SOLTU)
    Spacer
RNA interference cassette:
Transcription begins at the Solanum tuberosum granule bound starch synthase (gbss) promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript is contains (from 5' to 3'): a sense portion of gbss, gbss spacer region and an anti-sense portion of gbss. After transcription, the sense and anti-sense regions will base-pair to form a hairpin structure, which can elicit an RNA interference response. The spacer acts as a connecting loop to allow for the formation of this secondary structure. The transcript is not expected to be translated due to RNA interference cellular processing. For more information, kindly refer to the "Other gene(s) whose expression was affected by the transformation" section below.

Selectable marker:
Transcription of Arabidopsis thaliana acetohydroxyacid synthase commences from a nos promoter and terminates at a nos terminator.

Notes:
- Southern blot analysis indicated a single insertion of the T-DNA in the potato genome.
- The nos terminator of acetohydroxyacid synthase was truncated during transformation, but this deletion does not seem to affect the expression of the coding sequence. In the vector, the terminator was 253 basepairs.
EN
LMO characteristics
  • BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)
    Protein coding sequence | altered carbohydrate composition: increased amylopectin content
After transcription, the sense and antisense regions of the transcript will base-pair and form hairpin RNA (hpRNA). The double-strandedness is recognized by DICER, which will fragment the hpRNA into small interfering RNA (siRNA) of roughly 21 to 23 basepairs in size. These siRNAs will then complex with ARGONAUTE family proteins, which will unwind the siRNA duplex, leaving a single strand of the siRNA and activating the RISC complex. Using the complexed single strand of the siRNA, the RISC complex will seek out transcripts with complementarity to the siRNA. Transcripts with complementary sequences will be targetted degradation. Thus, the cellular RNAi machinery will target the endogenous granule bound starch synthase, resulting in the silencing of gene expression (reduction of mRNA and protein).
EN
  • Food
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 1
Country's Decision or any other Communication Living modified organism(s) 3
Risk Assessment generated by a regulatory process Living modified organism(s) 4
Living Modified Organism Related LMO(s) 3