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Living Modified Organism (LMO)
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BCH-LMO-SCBD-49073-10   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 08 Jul 2009 last updated: 13 Jul 2026
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
TREUS™, Plenish™ Soybean
EN
305423
Yes
DP-3Ø5423-1
  • - Organization: Pioneer Hi-Bred Production Inc. () | BCH-CON-CA-16116-1
    Organization:
    Pioneer Hi-Bred Production Inc. ()
    Phone:
    Fax:
    Email:
    Website:
The soybean (Glycine max) was modified for increased levels of monounsaturated fatty acid (oleic) and decreased levels of polyunsaturated fatty acids (linoleic and linolenic). The LM soybean was also modified for tolerance to sulfonylurea herbicides in order to be used as a marker selection for transformants. To achieve this trait, the soybean expresses the modified Glycine max microsomal omega-6 desaturase gene (fad2-1) where the fragment of the fad2-1 gene does not code for a protein and silences the expression of the endogenous soybean omega-6 desaturase, resulting in accumulated monounsaturated fats. For the selectable marker, to achieve tolerance to sulfonylurea, the soybean expresses the modified acetolactate synthase (als) gene from Glycine max (hra) which is a variant of the acetolactate synthase enzyme derived from Glycine max (also known as acetohydroxy acid synthase). Acetohydroxy acid synthase (also known as acetolactate synthase)  is a key enzyme that catalyzes the first common step in the biosynthesis of the essential branched-chain amino acids isoleucine, leucine, and valine. This protein contains two mutations in its amino acid sequence that reduce binding affinity to ALS-inhibiting herbicides and thus are responsible for GM-HRA resistance to ALS-inhibiting herbicides (e.g., sulfonylureas and triazolopyrimidines).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Secondary somatic embryos derived from explants of immature soybean seeds of the soybean ‘Jack' variety
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Characteristics of the modification process
PHP19340 and PHP17752
EN
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-103893-1 Kunitz trypsin inhibitor gene promoter | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Promoter
  • BCH-GENE-SCBD-103894-1 Kunitz trypsin inhibitor gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Terminator
  • BCH-GENE-SCBD-103895-3 SAMS Promoter | Glycine max (Soybean, Soya bean, Soya / soja /соя, SOYBN)
    Promoter
  • BCH-GENE-SCBD-100268-6 Acetohydroxy acid synthase gene | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Protein coding sequence | Resistance to herbicides (Sulfonylurea)
  • BCH-GENE-SCBD-103896-5 Acetohydroxy acid Synthase gene Terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Terminator
  • BCH-GENE-SCBD-115046-3 omega-6-desaturase | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
Information about the inserted DNA sequences
The DNA insert from DP-3Ø5423-1 comprised of two cassettes expressing a portion of the Glycine max omega-6-desaturase encoded by the fad2-1 gene fragment and a modified acetohydroxy acid synthase (als) encoded by the hra gene for sulfonylurea selection marker.

(1) The fragment of the gm-fad2-1 gene is under the control of the Glycine max Kunitz trypsin inhibitor gene (KTi3) promoter and the 3' untranslated region of the KTi3 gene (KTi3 terminator). Transcription of the gene fragment under the control of a seed-preferred KTi3 promoter acts to silence the expression of the endogenous soybean omega-6 desaturase.

(2) For the selection cassette, the hra gene is under the control of the G. max the S-adenosyl-L-methionine synthetase (SAMS) constitutive promoter and the als gene terminator.

Note
  • Microprojectile bombardment was used to co-transform secondary plant cell embryos with two purified linear DNA fragments: a 2924 base pair fragment (PHP19340A fragment) containing the gm-fad2-1 cassette, and the 4512 base pair fragment (PHP17752A fragment) containing the gm-hra cassette. 
  • The gm-fad2-1 gene fragment corresponds to approximately 40% of the middle portion of the fad2-1 gene (597 bp).
  • The gm-hra gene  is an optimized form of the endogenous als gene from soybean (1971 bp). The gm-hra gene has been modified by site directed mutagenesis (P183A and W560L) from the native soybean als gene and encodes a version of the enzyme that is tolerant to als inhibitors such as sulfonylurea herbicide. It also contains 15 additional nucleotides from the native als gene 5'UTR.
  • Southern blot and PCR analyses confirmed that the inserted DNA consists of four insertions that comprise:
    • Insertion 1: one truncated PHP19340A fragment with a truncated KTi3 terminator and intact gm-fad2-1 gene fragment and intact KTi3 promoter, one intact PHP19340A fragment, one intact PHP17752A fragment, one truncated PHP19340A fragment with an intact KTi3 promoter and a truncated gm-fad2-1 gene fragment, and one truncated PHP19340A fragment with a truncated KTi3 promoter and truncated gm-fad2-1 gene fragment.
    • Insertion 2: one truncated PHP19340A fragment with a truncated KTi3 promoter and with intact gm-fad2-1 gene fragment and intact KTi3 terminator.
    • Insertion 3: one truncated copy of the KTi3 promoter with a nonfunctional 495 bp fragment of the plasmid backbone; and
    • Insertion 4: two truncated PHP19340A fragments in an inverted repeat configuration, both with a truncated KTi3 promoter and intact gmfad2-1 gene fragment and KTi3 terminator.
  • The gm-fad2-1 cassette components present in the genome include: (1) 8 copies of the KTi3 promoter; (2) 7 copies of the gm-fad2-1 gene fragment; and (3) 5 copies of the KTi3 terminator.
  • The flanking genomic sequences and all insert DNA junctions were sequenced and analyzed for potential open reading frames (ORFs), and no novel proteins were predicted to be produced from any of the DNA junction sequences.
  • Southern blot analysis across three generations of self- and cross-pollinated plants demonstrated that the inserted DNA is stable across generations.
  • Only one recombination event was detected among approximately 1100 segregating individuals, indicating a very low frequency of rearrangement. PCR analysis confirmed that no additional recombinant individuals were present.
EN
LMO characteristics
EN
  • Biofuel
  • Food
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism or Parental Organisms 4
Living Modified Organism Related LMO(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 16
Country's Decision or any other Communication LMO identification 14
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 15

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