Detection and Identification Online Discussions 2019

Distinguished Colleagues,

The Detection and Identification Online Discussions 2019 provides an opportunity to update and share our experiences on current trends in the detection and identification of LMOs and emerging issues in the detection, identification and monitoring of organisms, components and products of synthetic biology. Also open for discussions are the current and existing capacities for the detection and identification of LMOs.
The feedback on these issues will be summarized as a resource for the network of laboratories for the detection and identification of LMOs.
We still have some time and therefore encourage meaningful engagements among participants. The Secretariat echoes the moderator’s invitation to participants to contribute to the discussions while thanking those that have done so already..

Best regards,
Shakirat Ajenifujah-Solebo

Dear participants,

A number of peer-reviewed sources of information are available in addition to the ENGL handbook.  Unlike regional handbooks, these resources discuss detection methods in the global context.  Several are open access.  For example, a discussion on sampling is available (Freese et al 2015) and a comparison of DNA and Protein-based detection methods (Alarcon, 2019) will be open access as of January 2020. Another good source of information is the book “Immunoassays in Agricultural Biotechnology“ edited by Guomin Shan (2010), which discusses the way that protein-based tests can be applied across the food chain.

Additionally the International Seed testing Association maintains a site with a list of sources of testing information at
https://www.seedtest.org/en/analytical-methods-_content---1--1477.html and links from this page. 

In response to the post of Apichart Vannavichit, [#10016] and Dr. Sarah Agapito-Tenfen [#10021], among others, the work of International Standards Organisation Technical Committee 34 subcommittee 16 (TC34/SC16) on detection methods is notable.  A number of ISO standards have been published that cover PCR and protein-based analytical methods, and work is in progress on a sampling standard.  The most relevant are listed below.

Citations:

-- Guomin Shan (Ed.) 2010.“Immunoassays in Agricultural Biotechnology”, John Wiley & Sons, Inc.   ISBN 978-0-470-28952-5

-- Clara M. Alarcon 2019. Application of DNA- and Protein-Based Detection Methods in Agricultural Biotechnology.  J Agric Food Chem. v67:1019-1028. doi: 10.1021/acs.jafc.8b05157.

-- Freese, L., Chen, J., and Shillito, R. Sampling of Grain and Seed to Estimate the Adventitious Presence of Biotechnology-Derived Seeds in a Lot. Cereal Foods World, v60, pp. 9-15, 2015.  DOI http://dx.doi.org/10.1094/CFW-60-1-0009

Relevant ISO TC34/SC16 standards – further information can be found at:  https://www.iso.org/committee/560239.html

-- ISO 16577: Molecular biomarker analysis -- Terms and definitions
-- ISO 24276: Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- General requirements and definitions
-- ISO 21569: Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Qualitative nucleic acid based methods
-- ISO 21570: Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Quantitative nucleic acid based methods
-- ISO 21571: Foodstuffs -- Methods of analysis for the detection of genetically modified organisms and derived products -- Nucleic acid extraction
-- ISO 21572: Foodstuffs -- Molecular biomarker analysis -- Protein-based methods
-- ISO/ TS 16393 Molecular biomarker analysis -- Determination of the performance characteristics of qualitative measurement methods and validation of methods
-- ISO 16578: Molecular biomarker analysis -- General definitions and requirements for microarray detection of specific nucleic acid sequences

The following standards are under development:

-- ISO/CD 20813: Molecular biomarker analysis -- Methods of analysis for the identification and the detection of animal species from foods and food products (Nucleic acid based methods) -- General requirements and definitions
-- ISO/AWI 22753: Molecular biomarker analysis -- Methods of analysis for the detection of genetically modified organisms -- Semi-quantitative method for the statistical evaluation of weight/weight GMO content in seeds and grains
-- ISO/NP 22942-1: Molecular biomarker analysis -- Nucleic acid isothermal nucleic acid amplification based methods -- Part 1: General requirements

Thank you Raymond for the resources you have shared with us. These links will be invaluable for forum participants.

More inputs are welcome please.


Shakirat