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Living Modified Organism
(LMO)
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Potato modified for altered phytochrome B expression
EN
DARA5 and DARA12
No
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Person:TU München, Lehrstuhl für Pflanzenzüchtung, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Technische Universität MünchenAlte Akademie 8Freising, Bayern
85354, GermanyPhone: +49 8161 71 -3422, +49 8161 71 -3258,Fax: +49 8161 71-3900,Related OrganizationTechnische Universität München ()Academic or research instituteAlte Akademie 8Freising, Bayern
85354, GermanyPhone: +49 8161 71 -3422, +49 8161 71 -3258,Fax: +49 8161 71-3900,
Potato plants are modified to consitutively express the phytochrome B gene from Arabidopsis thaliana which codes for a regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses.
As a result of the genetic modification, the anthocyanin and chlorophyll synthesis is enhanced and the photosynthesis rate is increased in the transgenic plants. Furthermore, a number of morphological and physiological changes take place, such as reduced apical dominance, shorter internodes, dwarfism, delayed senescence, thicker stems, starch deposits and increased specific weight in the stems and leaves, smaller leaves, elongation of the palisade parenchyma cells, enhanced root formation, increased number of tubers and increased tuber yield.
EN
As a result of the genetic modification, the anthocyanin and chlorophyll synthesis is enhanced and the photosynthesis rate is increased in the transgenic plants. Furthermore, a number of morphological and physiological changes take place, such as reduced apical dominance, shorter internodes, dwarfism, delayed senescence, thicker stems, starch deposits and increased specific weight in the stems and leaves, smaller leaves, elongation of the palisade parenchyma cells, enhanced root formation, increased number of tubers and increased tuber yield.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
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pMAB316::pTiB6S3-SE
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-111073-1 Phytochrome B gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in physiology and/or production (Growth rate, Photoperiod response, Yield),Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin), Pigmentation / Coloration)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-15171-5 Nopaline Synthase Gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Streptomycin)
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EN
- Research
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As a major transcription regulator in plants, phytochrome B (PhyB) affects the transcription of a variety of genes. Therefore, while not directly affected by the transformation, the regulation of genes involved in a variety of different physiological and morphological characteristics and metabolic pathways is altered by the elevated PhyB levels in the transgenic plants, such as the phytohormone balance, circadian regulation, regulation of flowering and seed germination, photosynthesis, phototropism and gravitropism, defense responses, the response to cold and the response to visible red and blue light.
EN
- UniProtKB - PhyB - P14713 (PHYB_ARATH) [ English ]
- UniProtKB - PhyB - P14713 (PHYB_ARATH) [ German ]
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