Potato modified for altered phytochrome B expression | BCH-LMO-SCBD-111078 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

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last updated: 20 Oct 2016
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Potato modified for altered phytochrome B expression
DARA5 and DARA12
Potato plants are modified to consitutively express the phytochrome B gene from Arabidopsis thaliana which codes for a regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses.

As a result of the genetic modification, the anthocyanin and chlorophyll synthesis is enhanced and the photosynthesis rate is increased in the transgenic plants. Furthermore, a number of morphological and physiological changes take place, such as reduced apical dominance, shorter internodes, dwarfism, delayed senescence, thicker stems, starch deposits and increased specific weight in the stems and leaves, smaller leaves, elongation of the palisade parenchyma cells, enhanced root formation, increased number of tubers and increased tuber yield.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-111073-1 Phytochrome B gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Protein coding sequence | Changes in physiology and/or production (Growth rate, Photoperiod response, Yield),Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin), Pigmentation / Coloration)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-15171-5 Nopaline Synthase Gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Streptomycin)
LMO characteristics
  • Research
Detection method(s)
Additional Information
As a major transcription regulator in plants, phytochrome B (PhyB) affects the transcription of a variety of genes. Therefore, while not directly affected by the transformation, the regulation of genes involved in a variety of different physiological and morphological characteristics and metabolic pathways is altered by the elevated PhyB levels in the transgenic plants, such as the phytohormone balance, circadian regulation, regulation of flowering and seed germination, photosynthesis, phototropism and gravitropism, defense responses, the response to cold and the response to visible red and blue light.
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1