MON-ØØ6Ø3-6 x ACS-ZMØØ3-2 - Roundup Ready™ Liberty Link™ maize | BCH-LMO-SCBD-100975 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ Liberty Link™ maize
NK603 x T25
The stacked maize line NK603 x T25 was produced by cross breeding between maize LMOs T25 and NK603 for tolerance to herbicides containing glufosinate and glyphosate.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
  • BCH-LMO-SCBD-14776-17 Living Modified Organism MON-ØØ6Ø3-6 - Roundup Ready™ maize
    Resistance to herbicides - Glyphosate
  • BCH-LMO-SCBD-14767-14 Living Modified Organism ACS-ZMØØ3-2 - Liberty Link™ maize
    Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
Characteristics of the modification process
PV-ZMGT32 and pDH51
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from NK603 vector PV-ZMGT32:

NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.

DNA insert from T25 vector pDH51:

Glufosinate tolerance in T25 maize is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
  • Food
  • Feed
  • Biofuel
Detection method(s)
NK603 x T25 was produced through cross breeding and contains the inserted genetic elements from both parents.

From the NK603 parent, NK603 x T25 contains one copy of a DNA cassette containing two cp4 epsps genes (see below). From the T25 parent, NK603 x T25 contains one copy of the DNA cassette with an intact Ori-pUC and the "P-35S - pat - T-35S" construct as well as a truncated bla gene (25% of the 5' end of the bla gene is missing).
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 11
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 3
Country's Decision or any other Communication Living modified organism(s) 10