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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato with altered starch composition
EN
AM02, lines AM02-1003, AM02-1005, AM02-1008, AM02-1010, AM02-1012, AM02-1014 and AM02-1017
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Person:BASF Plant Science GmbHCarl-Bosch-Str. 38Ludwigshafen,
67056 , GermanyPhone: +49 621 60-0,Fax: +49 621 60-42525,Email: global.info@basf.com,
AM02 potatos synthesize starch that is practically amylose-free and primarily consists of amylopectin.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
South America
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Potato with modified starch composition| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Imidazolinone, Sulfonylurea)
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Potato with altered starch composition| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio, Carbohydrates), Resistance to antibiotics (Kanamycin)
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Potato modified for altered starch composition| AVEBE | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Reduced amylose in starch content
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BPS-A1Ø2Ø-5 - Amadea potato| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Imidazolinone, Sulfonylurea), Selectable marker genes and reporter genes
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Potato with altered starch content| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Sulfonylurea)
pAP2
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
Solanum tuberosum was transformed with a construct containing a fragment of the coding region of the potato granule bound starch synthase (gbss) gene in antisense direction. By introduction of an antisense-sequence the edogeneous gbss gene is silenced post-transcriptionally. This leads to a significant reduced expression of GBSS-protein and therefore an impaired amylose synthesis; the synthesized starch within these potato lines consists almost exclusively of amylopectin.
As a selection marker a mutated version of the gene ahas (acetohydroxy acid synthase (AHAS), acetolactate synthase (ALS)) from Arabidopsis thaliana was choosen. The expression of this gene is controlled by the nos promoter and the ocs terminator from Agrobacterium tumefaciens. The AHAS protein catalyzes the first step of the biosynthesis of the amino acids valine, leucine and isoleucine and a homologous gene can be found in all plants. Unlike the native gene the used mutated version of the AHAS protein cannot be inhibited by the herbicide Imazamox (active ingriedient: sulfonylurea and/or imidazolinone).
EN
As a selection marker a mutated version of the gene ahas (acetohydroxy acid synthase (AHAS), acetolactate synthase (ALS)) from Arabidopsis thaliana was choosen. The expression of this gene is controlled by the nos promoter and the ocs terminator from Agrobacterium tumefaciens. The AHAS protein catalyzes the first step of the biosynthesis of the amino acids valine, leucine and isoleucine and a homologous gene can be found in all plants. Unlike the native gene the used mutated version of the AHAS protein cannot be inhibited by the herbicide Imazamox (active ingriedient: sulfonylurea and/or imidazolinone).
EN
- Research
PCR based methods, to be developed on information about the transformation vector.
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EN
- Molecular Basis of Imidazolinone Herbicide Resistance in Arabidopsis thaliana var Columbia.pdf [ English ]
- Molecular Basis of Imidazolinone Herbicide Resistance in Arabidopsis thaliana var Columbia.pdf [ German ]
- Herbicide resistant forms of Arabidopsis thaliana acetohydroxyacid synthase characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants..pdf [ English ]
- Herbicide resistant forms of Arabidopsis thaliana acetohydroxyacid synthase characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants..pdf [ German ]
- A novel selection system for potato transformation using a mutated AHAS gene [ English ]
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