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Living Modified Organism
(LMO)
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Barley modified for tolerance to drought and nutrient deficiencies
EN
pEXP:CKX2
No
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Organization:Palacky University in Olomouc ()Academic or research institute, Faculty of ScienceKrizkovskeho 8Olomouc,
771 47, Czech RepublicPhone: +420 585 634 060,Fax: +420 585 634 002,Email: dekanat.prf@upol.cz,Website: http://www.prf.upol.cz/en/,
The pEXP:CKX2 barley line has been genetically modified by inserting the Arabidopsis thaliana cytokinin dehydrogenase 2 gene under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice
Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, which are chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants. An important trait regulated by cytokinin is the size of the root system.
A reduced cytokinin status in plants (including barley) causes an enhanced root system which might render plants more tolerant to drought and nutrient deficiencies in the soil. Crop yield is often limited by the availability of water and soil-derived mineral nutrients. A larger root system may enable plants to gain access to more water and nutrients and in this way to cope with adverse environmental conditions.
EN
Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, which are chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants. An important trait regulated by cytokinin is the size of the root system.
A reduced cytokinin status in plants (including barley) causes an enhanced root system which might render plants more tolerant to drought and nutrient deficiencies in the soil. Crop yield is often limited by the availability of water and soil-derived mineral nutrients. A larger root system may enable plants to gain access to more water and nutrients and in this way to cope with adverse environmental conditions.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12110-5 Organism Hordeum vulgare (Barley, HORVU)Crops
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Barley modified for tolerance to drought and nutrient deficiencies| Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
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Barley modified for tolerance to drought and nutrient deficiencies| Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
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Barley modified for tolerance to drought and nutrient deficiencies| Palacky University in Olomouc | Resistance to antibiotics (Hygromycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought)
pEXP:CKX2
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-108705-1 LOC_Os04g11040.1 promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-108707-1 Cytokinin dehydrogenase 2 gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Tolerance to abiotic stress (Drought)
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
The transgenic line shows an up to 8-fold higher CKX enzyme activity in roots than the wild type. In contrast, CKX enzyme activity is similar to the control plants in leaves.
The cytokinin dehydrogenase 2 gene is under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice.
The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays polyubiquitin gene.
EN
The cytokinin dehydrogenase 2 gene is under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice.
The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays polyubiquitin gene.
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- Research
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EN
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