A stacked insect-resistant and herbicide-tolerant cotton derived by crossing MON-88913-8 with MON-15985-7 and MON-887Ø1-3, includes the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis subsp. kurstaki conferring resistance to lepidopteran pests, and the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase that confers tolerance to the herbicide glyphosate as well as Dicamba monooxygenase gene that confers tolerance to the herbicide dicamba and Phosphinothricin N-acetyltransferase gene that confers tolerance to the herbicide glufosate. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin and was used as a selectable marker.

DNA insert from MON 88913 vector PV-GHGT35
Roundup Ready® Flex cotton (MON 88913) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in cotton production. This genetically engineered cotton contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 88913 to survive otherwise lethal applications of glyphosate. The EPSPS gene introduced into MON 88913 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the EPSPS enzyme expressed by this gene is tolerant to glyphosate. MON 88913 cotton contains two copies of the EPSPS gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage.

DNA insert from MON15985 vector PV-GHBK11 and PV-GHBK04
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.

DNA insert from MON88701 vector PV-GHHT6997
Cotton modified with the insertion of transformation cassette containing the Dicamba monooxygenase (DMO) gene and Bialaphos resistance (BAR) gene to confer resistance to the herbicides Dicamba and Glufosinate respectively.

For additional information on this LMO, please refer to the records of the parental LMOs.