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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato modified for resistance against Potato Virus Y
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GPTV-Kan-NIb-blue Linda NIb36, GPTV-Kan-NIb-blue Linda NIb58, GPTV-Kan-NIb-blue Linda NIb60, GPTV-Kan-NIb-blue Linda NIb80, GPTV-Kan-NIb-blue Linda NIb88, DH59 NIb51, DH59 NIb93, DH59 NIb146, DH59 NIb156
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Person:BAZ, Institut für landwirtschaftliche KulturenErwin-Baur-Str. 27Quedlinburg,
06484, GermanyPhone: +49 (0)3946 47 701,Fax: +49 (0)3946 47 255,Email: zl@jki.bund.de,Website: http://www.jki.bund.de,Related OrganizationBundesanstalt für Züchtungsforschung an Kulturpflanzen (BAZ)Academic or research instituteErwin-Baur-Str. 27Quedlinburg,
06484, GermanyPhone: +49 (0)3946 47 701,Fax: +49 (0)3946 47 255,Email: zl@jki.bund.de,Website: http://www.jki.bund.de,
The genetically modified potato plants constitutively express a mutated fragment of the NIb gene of the Potato Virus Y, which, in its native form, codes for a RNA-dependent RNA polymerase. The modification is expected to confer resistance to PVY.
To enable the detection the Nib gene product, the modified NIb gene was fused to the enhanced blue fluorescent protein gene from the jellyfish Aequorea victoria as a reporter gene.
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To enable the detection the Nib gene product, the modified NIb gene was fused to the enhanced blue fluorescent protein gene from the jellyfish Aequorea victoria as a reporter gene.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
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Potato modified for resistance against Potato Virus Y| Bundesanstalt für Züchtungsforschung an Kulturpflanzen(BAZ) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Viruses, Potato virus Y (PVY)), Selectable marker genes and reporter genes
pGPTV-Kan-NIb-blue
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-111452-1 Nuclear inclusion protein b gene | Potato virus Y (PVY)Protein coding sequence | Resistance to diseases and pests (Viruses, Potato virus Y (PVY))
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BCH-GENE-SCBD-111453-1 Enhanced blue fluorescent protein gene | Aequorea victoria (Crystal Jellyfish, Water Jellyfish, AEQVI)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
A mutated sequence of the NIb replicase of the Potato Virus Y was fused to the enhanced blue fluorescent protein gene. They are expressed under the control of the 35S promoter and termination sequence of the Cauliflower Mosaic Virus.
The mutated sequence has a deletion at the C-terminus (approx. 400 nt) and at the N-terminus (6 nt), and an internal shift in the reading frame affecting 14 amino acids, leading to a non-functional gene product.
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The mutated sequence has a deletion at the C-terminus (approx. 400 nt) and at the N-terminus (6 nt), and an internal shift in the reading frame affecting 14 amino acids, leading to a non-functional gene product.
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- Research
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| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
| Living Modified Organism | Related LMO(s) | 2 | |
