Potato modified for resistance against Potato Virus Y | BCH-LMO-SCBD-111461 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 21 Nov 2016
Living Modified Organism identity
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Potato modified for resistance against Potato Virus Y
EN
GPTV-Kan-NIb-blue Linda NIb36, GPTV-Kan-NIb-blue Linda NIb58, GPTV-Kan-NIb-blue Linda NIb60, GPTV-Kan-NIb-blue Linda NIb80, GPTV-Kan-NIb-blue Linda NIb88, DH59 NIb51, DH59 NIb93, DH59 NIb146, DH59 NIb156
  • - Person: BAZ | BCH-CON-DE-101384-2
    Person
    BAZ
    , Institut für landwirtschaftliche Kulturen
    Erwin-Baur-Str. 27
    Quedlinburg,
    06484, Germany
    Phone: +49 (0)3946 47 701,
    Fax: +49 (0)3946 47 255,
    Email: zl@jki.bund.de,
    Related Organization
    Bundesanstalt für Züchtungsforschung an Kulturpflanzen (BAZ)
    Academic or research institute
    Erwin-Baur-Str. 27
    Quedlinburg,
    06484, Germany
    Phone: +49 (0)3946 47 701,
    Fax: +49 (0)3946 47 255,
    Email: zl@jki.bund.de,
The genetically modified potato plants constitutively express a mutated fragment of the NIb gene of the Potato Virus Y, which, in its native form, codes for a RNA-dependent RNA polymerase. The modification is expected to confer resistance to PVY.

To enable the detection the Nib gene product, the modified NIb gene was fused to the enhanced blue fluorescent protein gene from the jellyfish Aequorea victoria as a reporter gene.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • Potato modified for resistance against Potato Virus Y
    | BAZ Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Viruses - Potato virus Y (PVY) Selectable marker genes and reporter genes
Characteristics of the modification process
pGPTV-Kan-NIb-blue
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
A mutated sequence of the NIb replicase of the Potato Virus Y was fused to the enhanced blue fluorescent protein gene. They are expressed under the control of the 35S promoter and termination sequence of the Cauliflower Mosaic Virus.

The mutated sequence has a deletion at the C-terminus (approx. 400 nt) and at the N-terminus (6 nt), and an internal shift in the reading frame affecting 14 amino acids, leading to a non-functional gene product.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 2