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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant herbicide tolerant maize
EN
MZIR098
Yes
SYN-ØØØ98-3
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Person:Syngenta (Novartis (Ciba-Geigy))Phone:Fax:Email:Website:
Maize SYN-ØØØ98-3 was modified for resistance against coleopteran insects, particularly western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica berberi), and Mexican corn rootworm (Diabrotica vigifera zeae). The LM maize was also modified for tolerance to glufosinate-ammonium herbicides and used as a marker selection for transformants. To achieve resistance against coleopteran pests, the maize expresses the eCry3.1Ab and mCry3A genes which have a pore forming mode-of-action in the epithelial lining of feeding larvae; eCry3.1Ab and mCry3A produce a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. For the selectable marker, to achieve tolerance to glufosinate, the maize expresses expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Corn line: NP2222
EN
pSYN17629
EN
- Agrobacterium-mediated DNA transfer
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0.092 kb
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0.400 kb
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2.000 kb
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0.250 kb
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0.520 kb
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0.551 kb
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0.250 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-104789-2 eCry3.1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera), Northern corn rootworm (Diabrotica barberi))
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-43634-3 mCry3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles), Western corn rootworm (Diabrotica virgifera))
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-111966-2 Nopaline Synthase Enhancer | Agrobacterium tumefaciens (Agrobacterium)Leader
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BCH-GENE-SCBD-104788-2 Cestrum Yellow Leaf Curling Virus promoter | Cestrum yellow leaf curling virus (CYLCV)Promoter
Information on the inserted DNA sequences
The transforming plasmid for maize SYN-ØØØ98-3 contained a transfer DNA (T-DNA) region comprising three gene cassettes: two expressing insecticidal proteins, mCry3A derived from Bacillus thuringiensis and eCry3.1Ab, and one used for positive transformant selection, expressing the herbicide tolerance protein phosphinothricin N-acetyltransferase (PAT).
(1) The synthetic insecticidal protein eCry3.1Ab gene is regulated by the Cestrum yellow leaf curling virus promoter and the nopaline synthase terminator from Agrobacterium tumefaciens. A nopaline synthase enhancer from Agrobacterium tumefaciens was added to enhance the expression of eCry3.1Ab. Due to the presence of an enhancer and the constitutive nature of the promoter, high levels of transcription are expected.
(2) The mCry3A gene from Bacillus thuringiensis is regulated by the ubiquitin gene promoter from Zea mays and the nopaline synthase terminator from A. tumefaciens. High levels of transcription are expected due to the strong constitutive promoter.
(3) The pat gene from A. tumefaciens strain Tu494 is used as a selectable marker for transformants, and the gene is under the control of the Cauliflower mosaic virus promoter and the nopaline synthase terminator from A. tumefaciens. High levels of transcription are expected due to the strong constitutive promoter.
Note
The transforming plasmid for maize SYN-ØØØ98-3 contained a transfer DNA (T-DNA) region comprising three gene cassettes: two expressing insecticidal proteins, mCry3A derived from Bacillus thuringiensis and eCry3.1Ab, and one used for positive transformant selection, expressing the herbicide tolerance protein phosphinothricin N-acetyltransferase (PAT).
(1) The synthetic insecticidal protein eCry3.1Ab gene is regulated by the Cestrum yellow leaf curling virus promoter and the nopaline synthase terminator from Agrobacterium tumefaciens. A nopaline synthase enhancer from Agrobacterium tumefaciens was added to enhance the expression of eCry3.1Ab. Due to the presence of an enhancer and the constitutive nature of the promoter, high levels of transcription are expected.
(2) The mCry3A gene from Bacillus thuringiensis is regulated by the ubiquitin gene promoter from Zea mays and the nopaline synthase terminator from A. tumefaciens. High levels of transcription are expected due to the strong constitutive promoter.
(3) The pat gene from A. tumefaciens strain Tu494 is used as a selectable marker for transformants, and the gene is under the control of the Cauliflower mosaic virus promoter and the nopaline synthase terminator from A. tumefaciens. High levels of transcription are expected due to the strong constitutive promoter.
Note
- The gene eCry3.1Ab is a fusion between the 5′ end of a modified Cry3A gene (mCry3A) and the 3′ end of a synthetic Cry1Ab gene.
- Molecular characterization of MZIR098 was carried out using nucleotide sequencing and Southern blot analyses.
- These analyses indicated that a single copy of the T-DNA was inserted in to the genome of the recipient organism and that and it does not contain the plasmid backbone sequence from transformation plasmid pSYN17629.
- Sequence analysis of the MZIR098 insertion site demonstrated that 24-bp from the corn genomic sequence was deleted during the integration of the MZIR098 insert.
EN
- Food
- SYN-ØØØ98-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
EN
EN
- EUginius: MZIR098 [URL] [ English ]
- OECD Biotrack database: MZIR098 [URL] [ English ]
- ISAAA GM Approval database: MZIR098 [URL] [ English ]
- FSANZ Application. Food derived from Herbicide-tolerant & Insect-protected Corn Line MZIR098 (A1116) [PDF] ( Food Standards Australia New Zealand ) [ English ]
- Pest Management Science (2023). Bt corn hybrids expressing mCry3A and eCry3.1Ab Proteins protect corn roots against western corn rootworm injury [DOI] [ English ]
- FAO GM Foods Platform: SYN-ØØØ98-3 [URL] [ English ]
- CFIA: Novel food information - Herbicide-tolerant and insect-protected corn event MZIR098 [URL] [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Recipient Organism or Parental Organisms | 2 | |
| Country's Decision or any other Communication | LMO identification | 13 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 13 | |
| Laboratory for detection and identification of LMOs | LMO(s) detectable by the laboratory | 3 | |
