BCS-GHØØ2-5 × MON-15985-7 - Herbicide-tolerant, insect-resistant cotton | BCH-LMO-SCBD-111987 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 05 Jun 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant cotton
EN
GHB614 x MON15985
Yes
BCS-GHØØ2-5 × MON-15985-7
The stacked cotton line  was generated through the traditional cross breeding of each of parental organisms to express the cry1A(c), cry2A(b), and 2mepsps genes. The expression of these genes confers resistance against lepidoptera pests and tolerance to glyphosate herbicides.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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Characteristics of the modification process
PV-GHBK11, PV-GHBK04 and pTEM2
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Terminator
  • BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)
    Leader
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-104647-3 Histone H4 gene Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-104648-2 Histone H3 Gene II intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-101419-4 Optimized Transit Peptide
    Transit signal
  • BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-104646-4 Histone H4 gene 3' UTR | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Terminator
DNA insert from 15985 vector PV-GHBK11
The T-DNA from this vector contributed the coding sequences of the cry2A(b) and uidA genes. These allow for the expression of the insecticidal cry2A(b) crystal protein which protects against lepidoptera pests and the Beta-Glucuronidase selectable marker.

DNA insert from 15985 vector PV-GHBK04
Originally integrated into the MON531 line and persisted through to the 15985 line. The T-DNA from this vector contains the coding sequence of the cry1A(c)  gene and the nptII resistance gene. The aadA gene was also integrated into the host genome however it is not expressed.

DNA insert from GHB614 vector pTEM2
Cotton event GHB614 contains a stably integrated 2mepsps gene. This modification confers to the protein a decreased binding affinity for the herbicide glyphosate, allowing it to maintain sufficient enzymatic activity and thus tolerance in its presence.

For additional information on this LMO, please refer to the records of the parental LMOs.
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LMO characteristics
EN
  • Food
  • Feed
  • Fiber/textile
Additional Information
EN