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Living Modified Organism (LMO)
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Viral resistant cassava with increased levels of zinc and iron
EN
D9001-914004
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OrganizationNational Root Crops Research Institute (NRCRI)Academic or research instituteKm 8 Ikot Ekpene Road, PMB 7006Umuahia, Abia
, NigeriaPhone: +234 (0)8168983790, +2349035068714,Fax:Website: https://nrcri.gov.ng/,
Cassava (Manihot esculenta Crantz) was modified to express Arabidopsis thaliana FERRITIN 1 and IRON-REGULATED TRANSPORTER 1 to increase the levels of iron and zinc. The modified cassava additionally contains an RNAi cassette to target the coat proteins of Cassava Brown Streak Virus and Ugandan Cassava Brown Streak Virus and convey resistance to these viruses, the causal agents of Cassava brown streak disease.
Previous overepxression of FER1 and IRT1 increased iron and zinc levels by 7-18 times 3-10 times, respectively.
A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
Previous overepxression of FER1 and IRT1 increased iron and zinc levels by 7-18 times 3-10 times, respectively.
A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-112539-1 Organism Manihot esculenta (Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES)Crops
Cassava cultivar: TMS 91/02324
p9001
EN
- Agrobacterium-mediated DNA transfer
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2.760 kb
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Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101900-6 CsVMV promoter | Cassava vein mosaic virus (Cassava vein mosaic virus, CVMV, CsVMV)Promoter
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BCH-GENE-SCBD-115108-2 Coat protein | Cassava brown streak virus (Cassava Brown Streak Virus, CBSV)Double-stranded RNA
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BCH-GENE-SCBD-115110-2 Coat Protein | Ugandan cassava brown streak virus (UCBSV)Double-stranded RNA
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BCH-GENE-SCBD-103123-6 Pyruvate orthophosphate dikinase, Intron 3 | Flaveria trinervia (Clustered Yellowtops, speedyweed, flaveria, yellow twinstem)Intron
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene TerminatorTerminator
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BCH-GENE-SCBD-115111-1 Phloem protein 2-A14 promoter | (Thale cress)Promoter
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BCH-GENE-SCBD-115112-1 Iron-regulated transporter 1 | (Thale cress)Protein coding sequence | Changes in quality and/or metabolite content,Increased levels of iron
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BCH-GENE-SCBD-115113-1 Phloem protein 2-A14 3' untranslated region | (Thale cress)Terminator
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BCH-GENE-SCBD-100273-4 B33 gene promotor | (Potato)Promoter
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BCH-GENE-SCBD-115136-1 Ferritin 1 | (Thale cress)Protein coding sequence | Changes in quality and/or metabolite content,Increased levels of iron,Tolerance to abiotic stress,Tolerance to excess iron
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BCH-GENE-SCBD-115137-1 Patatin-1 3' untranslated region | (Potato)Terminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | (Bacteria)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoterPromoter
RNA interference cassette:
Transcription of the cassette produces a hairpin RNA (hpRNA). RNA polymerase is recruited to the Cassava vein mosaic virus promoter and then transcribes: two segments of Cassava Brown Streak Virus (CBSV) and the Ugandan Cassava Brown Streak Virus (UCBSV) coat protein (sense orientation), the Flaveria trinervia pyruvate orthophosphate dikinase intron 3 (PDK), and CBSV and UCBSV coat protein (anti-sense orientation). Transcription stops at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The produced RNA then can form a double stranded segment due to the interstrand homology between the CBSV and UCBSV coat protein segments and the flexible linker/loop, PDK. The double stranded segment of the hpRNA is sufficient to trigger an RNA interference (RNAi) response. The host cell machinery then processes the hpRNA into small interfering RNA, which is used to guide the targeted degradation of RNA molecules with sequence homology. In this case, infecting viral transcripts are degraded by the host cell.
Iron-regulated transporter cassette:
Transcription of the Arabidopsis thaliana iron-regulated transporter 1 is under control of the A. thaliana phloem protein 2-A14 promoter and terminator.
Ferritin-1 cassette:
Arabidopsis thaliana ferritin-1 is under transcriptional control of the Solanum tuberosum patatin-1 promoter and terminator.
Selectable marker cassette:
Transcription of the Escherichia coli neomycin phosphotransferase II occurs from the duplicated enhanced Cauliflower Mosaic Virus 35S promoter and terminates at the nos terminator.
Please note all genetic elements were in the anti-sense orientation.
Transcription of the cassette produces a hairpin RNA (hpRNA). RNA polymerase is recruited to the Cassava vein mosaic virus promoter and then transcribes: two segments of Cassava Brown Streak Virus (CBSV) and the Ugandan Cassava Brown Streak Virus (UCBSV) coat protein (sense orientation), the Flaveria trinervia pyruvate orthophosphate dikinase intron 3 (PDK), and CBSV and UCBSV coat protein (anti-sense orientation). Transcription stops at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The produced RNA then can form a double stranded segment due to the interstrand homology between the CBSV and UCBSV coat protein segments and the flexible linker/loop, PDK. The double stranded segment of the hpRNA is sufficient to trigger an RNA interference (RNAi) response. The host cell machinery then processes the hpRNA into small interfering RNA, which is used to guide the targeted degradation of RNA molecules with sequence homology. In this case, infecting viral transcripts are degraded by the host cell.
Iron-regulated transporter cassette:
Transcription of the Arabidopsis thaliana iron-regulated transporter 1 is under control of the A. thaliana phloem protein 2-A14 promoter and terminator.
Ferritin-1 cassette:
Arabidopsis thaliana ferritin-1 is under transcriptional control of the Solanum tuberosum patatin-1 promoter and terminator.
Selectable marker cassette:
Transcription of the Escherichia coli neomycin phosphotransferase II occurs from the duplicated enhanced Cauliflower Mosaic Virus 35S promoter and terminates at the nos terminator.
Please note all genetic elements were in the anti-sense orientation.
The hpRNA cassette containing the coat proteins of CBSV and UCBSV is expected to be processed into small interfering RNA between 21-24 nucleotides in length. Due to this processing, proteins are not expected to be produced from this transcript.
- Food
- Research
Please note:
Transformation event is a placeholder. The following transformation events are expected to have similar genetics: D9001-914004, D9001-914005, D9001-914006, D9001-914010, D9001-914012, D9001-914017, D9001-914018, D9001-914019, D9001-914022, D9001-914023, D9001-914026, D9001-914028 and D9001-914029.
This field will be updated as more information is provided and once an event has been chosen for commercialization.
Transformation event is a placeholder. The following transformation events are expected to have similar genetics: D9001-914004, D9001-914005, D9001-914006, D9001-914010, D9001-914012, D9001-914017, D9001-914018, D9001-914019, D9001-914022, D9001-914023, D9001-914026, D9001-914028 and D9001-914029.
This field will be updated as more information is provided and once an event has been chosen for commercialization.
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