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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Banana modified for Xanthomonas wilt disease resistance
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PFLP
No
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Nairobi,Person:Dr. Leena TripathiPrincipal Scientist,
, KenyaPhone: +254 208632900,Fax:Email: l.tripathi@cgiar.org,Website:Related OrganizationInternational Institute of Tropical Agriculture, Kenya (IITA)Academic or research institute
The banana was modified for resistance to Xanthomonas wilt disease caused by Xanthomonas campestris pv. musacearum. The modified banana expresses Capsicum annuum plant ferredoxin-like protein (pflp), which intensifies the host plant's hypersensitive response triggered by invading bacterial pathogens and leads to an increased production of reactive oxygen species. The modified banana plants did not demonstrate wilting when challenged with X. campestris. In addition to pflp, the modified banana also contains a selectable marker, Escherichia coli neomycin phosphotransferase II, for kanamycin selection during transformation.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-100304-3 Organism Musa sp. (Banana, Plantain)Crops
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Banana resistant to Xanthomonas wilt disease| International Institute of Tropical Agriculture, Kenya(IITA) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Bacteria), Resistance to Xanthomonas sp., Selectable marker genes and reporter genes
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Banana modified for Xanthomonas wilt disease resistance| International Institute of Tropical Agriculture, Kenya(IITA) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Bacteria), Resistance to Xanthomonas sp., Selectable marker genes and reporter genes
pBI-PFLP
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- Agrobacterium-mediated DNA transfer
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0.000 kb
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0.180 kb
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0.795 kb
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0.253 kb
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0.346 kb
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0.432 kb
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0.253 kb
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0.000 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-115600-1 Ferredoxin-like amphipathic protein | Capsicum annuum (Bell pepper, Sweet pepper, Chili pepper, CAPAN)Protein coding sequence | Resistance to diseases and pests (Bacteria)
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
Gene expression
Transcription of Escherichia coli neomycin phosphotransferase II is under control of the Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator.
Transcription of Capsicum annuum plant ferredoxin-like protein (pflp) is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the nos terminator. Elevated levels transcription are expected due to the constitutive nature of the CaMV promoter.
Notes:
- The coding sequence of pflp is a cDNA copy of the natural transcript and thus introns are not present.
- PFLP contains an N-terminal peptide for localization to the chloroplasts of the cells. However, research has demonstrated that the transgenic protein may also be present in the cytoplasm.
- The transformation vector was derived from the pBI121 vector using a BamHI and SacI restriction digest.
EN
Transcription of Escherichia coli neomycin phosphotransferase II is under control of the Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator.
Transcription of Capsicum annuum plant ferredoxin-like protein (pflp) is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the nos terminator. Elevated levels transcription are expected due to the constitutive nature of the CaMV promoter.
Notes:
- The coding sequence of pflp is a cDNA copy of the natural transcript and thus introns are not present.
- PFLP contains an N-terminal peptide for localization to the chloroplasts of the cells. However, research has demonstrated that the transgenic protein may also be present in the cytoplasm.
- The transformation vector was derived from the pBI121 vector using a BamHI and SacI restriction digest.
EN
- Food
- Research
Some DNA and protein -based detection methodologies can be found in the scientific publication detailing the development of the banana event.
EN
EN
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