Banana resistant to Xanthomonas wilt disease | BCH-LMO-SCBD-115604 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 11 Jun 2020
Living Modified Organism identity
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Banana resistant to Xanthomonas wilt disease
EN
HRAP-PFLP
No
The modified banana was a result of a stacked event and expresses Capsicum annuum plant ferredoxin-like protein and hypersensitive response-assisting protein for resistance to the bacterium Xanthomonas campestris pv. musacearum, the causal agent of Xanthomonas wilt disease. Both proteins intensify the hypersensitive response and increase reactive oxygen species in response to the pathogen associated molecular pattern, harpinPSS, and thus confer resistance to Gram negative bacterial pathogens, such as X. campestris. When challenged with X. campestris during glasshouse experiments, the modified banana plants did not exhibit wilting or signs of successful infection. In addition, the modified banana also contains a selectable marker, Escherichia coli neomycin phosphotransferase II, for kanamycin selection during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • BCH-LMO-SCBD-115602-1
    Banana modified for Xanthomonas wilt disease resistance
    | International Institute of Tropical Agriculture, Kenya(IITA) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Bacteria), Resistance to Xanthomonas sp., Selectable marker genes and reporter genes
    Show detection method(s)
  • BCH-LMO-SCBD-115603-1
    Banana modified for Xanthomonas wilt disease resistance
    | International Institute of Tropical Agriculture, Kenya(IITA) | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Bacteria), Resistance to Xanthomonas sp., Selectable marker genes and reporter genes
    Show detection method(s)
Characteristics of the modification process
pBI-HRAP-PFLP (derived from pBI121)
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-113355-1 Hypersensitive response assisting protein gene | Capsicum annuum (Bell pepper, Sweet pepper, Chili pepper, CAPAN)
    Protein coding sequence | Resistance to diseases and pests
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-115600-1 Ferredoxin-like amphipathic protein | Capsicum annuum (Bell pepper, Sweet pepper, Chili pepper, CAPAN)
    Protein coding sequence | Resistance to diseases and pests (Bacteria)
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
Gene expression
Transcription of Escherichia coli neomycin phosphotransferase II is under control of the Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator. (clockwise direction)

Transcription of Capsicum annuum plant ferredoxin-like protein (pflp) is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the nos terminator. (counterclockwise direction)

Transcription of C. annuum hypersensitive response assisting protein (hrap) is under control of the CaMV 35S promoter and the nos terminator. (clockwise direction)

Note:
- The coding sequence of pflp and hrap are cDNA copies of the natural transcript and thus introns are not present.
- Elevated levels of the Pflp and Hrap are expected due to the constitutive nature of the CaMV promoter.
- PFLP contains an N-terminal peptide for localization to the chloroplasts of the cells.
- The transformation vector was derived from the pBI-HRAP vector, which was obtained by using a BamHI and SacI restriction digest to remove the GUS reporter. A further HindIII digest allowed for the insertion of the pflp cassette in the opposite orientation.
EN
LMO characteristics
EN
  • Food
  • Research
Detection method(s)
Scientific publications detailing the development of this event and related events (HRAP and PFLP) contain information regarding the detection of the inserted elements.
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 2

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