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Living Modified Organism (LMO)
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Moonburst™ carnation
EN
406191
Yes
FLO-4Ø62Ø-9
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Melbourne, VICPerson:Stephen ChandlerCosnultant,
, AustraliaPhone: +61 409 387 386,Fax:Email: schandler@florigene.com.au,Website: http://www.florigene.com,Related OrganizationSUntory Holdings Ltd. ()Private sector (business and industry)Melbourne, VIC
, AustraliaPhone: +61 409 387 386,Fax:Email: schandler@florigene.com.au,Website: http://www.florigene.com,
The modified carnation (Dianthus caryophyllus) was vegetatively propagated from the parental variety Moonshade™ and differs from the parent in having a flecked, bi-colour flower colour pattern. The carnation contains Petunia hybrida flavonoid 3'5' hydroxylase and dihydroflavonol-4 reductase, which together promote biosynthesis of delphinidin and anthocyanin pigments. The accumulation of pigments results in lavendar-coloured flowers. The modified carnation additionally contains Nicotiania tabacum acetolactate synthase for chlorsulfuron selection during tissue culturing.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-4954-7 Organism Dianthus caryophyllus (Carnation, DIACA)Crops
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BCH-LMO-SCBD-14828-9 Living Modified Organism FLO-4Ø619-8 - Moonshade™ carnationSUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea), Selectable marker genes and reporter genes
Moonburst™ carnation is a clone of the FLORIGENE Moonshade™ carnation.
The parental Moonshade carnation was sourced from the "UNESCO White" variety (cultivar FE 123), which contains a mutation in DFR and results in a non-functional enzyme (resulting in white flowers).
EN
The parental Moonshade carnation was sourced from the "UNESCO White" variety (cultivar FE 123), which contains a mutation in DFR and results in a non-functional enzyme (resulting in white flowers).
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FLO-4Ø686-3 - Moonstrike™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
pCGP1470
EN
- Agrobacterium-mediated DNA transfer
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0.190 kb
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0.060 kb
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3.761 kb
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0.000 kb
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1.157 kb
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1.520 kb
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0.818 kb
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1.225 kb
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1.142 kb
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0.717 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)Leader
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BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
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BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)Promoter
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BCH-GENE-SCBD-15010-3 Flavonoid 3', 5' hydroxylase gene | Petunia hybrida (Petunia, PETHY)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)Terminator
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BCH-GENE-SCBD-103773-1 mac-1 PromoterPromoter
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BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-103774-1 Mannopine synthase gene terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
Gene expression
The T-DNA insertion contained three gene cassettes: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida flavonoid 3', 5' hydroxylase (F3'5'H) and P. hybrida dihydroflavonol-4-reductase (DFR).
Transcription of ALS is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and the ALS terminator. A 5' untranslated leader from P. hybrida chlorophyll a/b-binding protein is also transcribed, but is not expected to be translated. The leader sequence augments the level of transcription (enhances the expression) of ALS.
Transcription of F3'5'H is under control of the Antirrhinum majus chalcone synthase gene promoter and the P. hybrida D8 terminator.
Transcription of DFR is under control of the synthetic Mac-1 promoter and the Agrobacterium tumefaciens mannopine synthase gene terminator.
Note:
- The genetic element size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of coding sequence + terminator)
- The Mac-1 promoter is a synthetic promoter compromised of A. tumefaciens mas promoter and CaMV enhancer sequences.
For more information, kindly refer to the parental record.
EN
The T-DNA insertion contained three gene cassettes: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida flavonoid 3', 5' hydroxylase (F3'5'H) and P. hybrida dihydroflavonol-4-reductase (DFR).
Transcription of ALS is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and the ALS terminator. A 5' untranslated leader from P. hybrida chlorophyll a/b-binding protein is also transcribed, but is not expected to be translated. The leader sequence augments the level of transcription (enhances the expression) of ALS.
Transcription of F3'5'H is under control of the Antirrhinum majus chalcone synthase gene promoter and the P. hybrida D8 terminator.
Transcription of DFR is under control of the synthetic Mac-1 promoter and the Agrobacterium tumefaciens mannopine synthase gene terminator.
Note:
- The genetic element size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of coding sequence + terminator)
- The Mac-1 promoter is a synthetic promoter compromised of A. tumefaciens mas promoter and CaMV enhancer sequences.
For more information, kindly refer to the parental record.
EN
- Ornamental
EN
EN
- FLORIGENE® Moonburst™ [ English ]
- Pigment biosynthesis in flowers.docx [ English ]
- Flower colour and cytochromes P450.pdf [ English ]
- EUginius - FLO-4Ø619-8 (parental variety) [ English ]
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