FLO-4Ø62Ø-9 - Moonburst™ carnation | BCH-LMO-SCBD-115777 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
last updated: 23 Nov 2020
Living Modified Organism identity
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Moonburst™ carnation
The modified carnation (Dianthus caryophyllus) was vegetatively propagated from the parental variety Moonshade™ and  differs from the parent in having a flecked, bi-colour flower colour pattern. The carnation contains Petunia hybrida flavonoid 3'5' hydroxylase and dihydroflavonol-4 reductase, which together promote biosynthesis of delphinidin and anthocyanin pigments. The accumulation of pigments results in lavendar-coloured flowers. The modified carnation additionally contains Nicotiania tabacum acetolactate synthase for chlorsulfuron selection during tissue culturing.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-4954-7 Organism Dianthus caryophyllus (Carnation, DIACA)
  • BCH-LMO-SCBD-14828-9 Living Modified Organism FLO-4Ø619-8 - Moonshade™ carnation
    SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea), Selectable marker genes and reporter genes
Moonburst™ carnation is a clone of the FLORIGENE Moonshade™ carnation.

The parental Moonshade carnation was sourced from the "UNESCO White" variety (cultivar FE 123), which contains a mutation in DFR and results in a non-functional enzyme (resulting in white flowers).
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
  • BCH-GENE-SCBD-15010-3 Flavonoid 3', 5' hydroxylase gene | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
  • BCH-GENE-SCBD-103773-1 mac-1 Promoter
  • BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-103774-1 Mannopine synthase gene terminator | Agrobacterium tumefaciens (Agrobacterium)
Gene expression
The T-DNA insertion contained three gene cassettes: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida flavonoid 3', 5' hydroxylase (F3'5'H) and P. hybrida dihydroflavonol-4-reductase (DFR).

Transcription of ALS is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and the ALS terminator. A 5' untranslated leader from P. hybrida chlorophyll a/b-binding protein is also transcribed, but is not expected to be translated. The leader sequence augments the level of transcription (enhances the expression) of ALS.

Transcription of F3'5'H is under control of the Antirrhinum majus chalcone synthase gene promoter and the P. hybrida D8 terminator.

Transcription of DFR is under control of the synthetic Mac-1 promoter and the Agrobacterium tumefaciens mannopine synthase gene terminator.

- The genetic element size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of coding sequence + terminator)
- The Mac-1 promoter is a synthetic promoter compromised of A. tumefaciens mas promoter and CaMV enhancer sequences.

For more information, kindly refer to the parental record.
LMO characteristics
  • Ornamental
Detection method(s)
Additional Information
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