Bt rice | BCH-LMO-SCBD-115916 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 24 Feb 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Bt rice
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PE-7
  • - Organization: Maharashtra Hybrid Seeds Company Limited (Mahyco) | BCH-CON-SCBD-115887-1
    Organization
    Maharashtra Hybrid Seeds Company Limited (Mahyco)
    Private sector (business and industry)
    , Head Office
    Jalna - Aurangabad Road, Post Box no. 76, Dawalwadi, Jalna
    Dawalwadi, Maharashtra
    431203, India
    Phone: +91 02482-262471,
    Fax: +91 02482-26002,
    Email: info@mahyco.com,
The rice (Oryza sativa) was modified for resistance to Lepidoptera pests (e.g. yellow stem borer) through the expression of Bacillus thuringiensis Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the rice contains an Escherichia coli hygromycin B phosphotransferase cassette for hygromycin selection during transformation and beta-glucuronidase as a reporter.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Rice line MPH-I
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  • Bt rice
    | Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
  • Bt rice
    | Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Characteristics of the modification process
pMH0102 and pCAMBIA1201
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  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The rice was modified using Agrobacterium-mediated transformation. The Rhizobium radiobacter (Agrobacterium) contained two transformation plasmids pMH0102 (Bacillus thuringiensis crystal protein cry1Ab) and pCAMBIA1201 (Escherichia coli hygromycin B phosphotransferase (hpt) and E. coli beta-glucuronidase (gus)).

Transcription of  cry1Ac starts from the Cauliflower mosaic virus (CaMV) enhanced 35S promoter and ends at a terminator. Transcription is expected to occur at elevated levels due to the nature of the CaMV promoter.

Transcription of hpt is under control of a CaMV enhanced 35S promoter and CaMV 35S terminator. Due to the nature of the CaMV promoter, transcription is expected to occur at elevated levels.

Transcription of gus is under control of a CaMV 35S promoter and a R. radiobacter nopaline synthase terminator. The gus sequence has a Ricinus communis catalase 1 intron at the 5' of the coding sequence for expression in plant, but not bacterial systems. The CaMV promoter induces high levels of transcription.

Note:
- Segregation analysis indicated that the genome contains a single insertion of the cry1Ac coding sequence.
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LMO characteristics
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  • Food
Detection method(s)
Kindly refer to the attached patent in the 'Additional information' section below.
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Additional Information
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