DAS-Ø15Ø7-1 × MON-ØØ6Ø3-6 - Herculex™ I Roundup Ready™ 2 maize | BCH-LMO-SCBD-14808 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 23 Jul 2013
Living Modified Organism identity
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Herculex™ I Roundup Ready™ 2 maize
TC1507 x NK603
DAS-Ø15Ø7-1 × MON-ØØ6Ø3-6
This LMO is the result of a cross between LMOs DAS-Ø15Ø7-1 and MON-ØØ6Ø3-6.

The MON-ØØ6Ø3-6 line of maize was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.

Corn line DAS-Ø15Ø7-1 is modified with the insertion of the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
  • BCH-LMO-SCBD-14841-13 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maize
    Dow AgroSciences, Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-14776-17 Living Modified Organism MON-ØØ6Ø3-6 - Roundup Ready™ maize
    Monsanto | Resistance to herbicides (Glyphosate)
Characteristics of the modification process
PHP8999 and PV-ZMGT32
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Notes about the Other(s) sequence(s) specific to this LMO

DNA construct from TC1507:

P-ubiZM1 (1.98) >> cry1F (1.82) >> T-ORF25 (0.72)* | P-35S (0.55) >> pat (0.55) >> T-35s (0.2)

DNA construct from NK603:

P-Ract1 >> I-Ract1 >> TS-CTP2 >> cp4-epsps >> T-nos | spacer (217) |  P-e35S >> I-Hsp70  >> TS-CTP2 >> cp4-epsps >> T-nos

Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.


* The EFSA document attached below indicates that the 3’ sequence from the Agrobacterium tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
LMO characteristics
  • Food
  • Feed
  • Biofuel