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Living Modified Organism (LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herculex™ I Roundup Ready™ 2 maize
EN
TC1507 x NK603
Yes
DAS-Ø15Ø7-1 × MON-ØØ6Ø3-6
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Organization:Dow AgroSciences ()Phone:Fax:Email:
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Organization:Pioneer Hi-Bred International Inc. ()Private sector (business and industry)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, United States of AmericaPhone: +1 515 535-3200,Fax:Email:Website: www.pioneer.com/,
This LMO is the result of a cross between LMOs DAS-Ø15Ø7-1 and MON-ØØ6Ø3-6.
The MON-ØØ6Ø3-6 line of maize was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.
Corn line DAS-Ø15Ø7-1 is modified with the insertion of the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.
EN
The MON-ØØ6Ø3-6 line of maize was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.
Corn line DAS-Ø15Ø7-1 is modified with the insertion of the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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BCH-LMO-SCBD-14841-13 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maizeDow AgroSciences, Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
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BCH-LMO-SCBD-14776-17 Living Modified Organism MON-ØØ6Ø3-6 - Roundup Ready™ maizeMonsanto | Resistance to herbicides (Glyphosate)
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PHP8999 and PV-ZMGT32
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- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
Notes about the Other(s) sequence(s) specific to this LMO
DNA construct from TC1507:
P-ubiZM1 (1.98) >> cry1F (1.82) >> T-ORF25 (0.72)* | P-35S (0.55) >> pat (0.55) >> T-35s (0.2)
DNA construct from NK603:
P-Ract1 >> I-Ract1 >> TS-CTP2 >> cp4-epsps >> T-nos | spacer (217) | P-e35S >> I-Hsp70 >> TS-CTP2 >> cp4-epsps >> T-nos
Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.
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* The EFSA document attached below indicates that the 3’ sequence from the Agrobacterium tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
EN
DNA construct from TC1507:
P-ubiZM1 (1.98) >> cry1F (1.82) >> T-ORF25 (0.72)* | P-35S (0.55) >> pat (0.55) >> T-35s (0.2)
DNA construct from NK603:
P-Ract1 >> I-Ract1 >> TS-CTP2 >> cp4-epsps >> T-nos | spacer (217) | P-e35S >> I-Hsp70 >> TS-CTP2 >> cp4-epsps >> T-nos
Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.
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* The EFSA document attached below indicates that the 3’ sequence from the Agrobacterium tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
EN
- Food
- Feed
- Biofuel
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-ØØ6Ø3-6 - CropLife International Detection Methods Database ( CropLife ) [ English ]
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