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Living Modified Organism (LMO)
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Moonlite™ carnation
EN
123.2.38 (40644)
Yes
FLO-4Ø644-6
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Person:Stephen ChandlerCosnultant,
, AustraliaPhone: +61 409 387 386,Fax:Email: schandler@florigene.com.au,Website: http://www.florigene.com,Related OrganizationSUntory Holdings Ltd. ()Private sector (business and industry)Melbourne, VIC
, AustraliaPhone: +61 409 387 386,Fax:Email: schandler@florigene.com.au,Website: http://www.florigene.com,
Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida. A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
NOTE: This LMO was formerly referred to with the UID FLO-4Ø644-4.
EN
NOTE: This LMO was formerly referred to with the UID FLO-4Ø644-4.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-4954-7 Organism Dianthus caryophyllus (Carnation, DIACA)Crops
EN
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FLO-Ø7442-5 - Moondust™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
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FLO-4Ø619-8 - Moonshade™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea), Selectable marker genes and reporter genes
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FLO-ØØØ15-3 - Moondust™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
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FLO-ØØØ16-4 - Moondust™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
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FLO-ØØØØ4-1 - Moondust™ carnation| SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
pCGP1470
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15010-3 Flavonoid 3', 5' hydroxylase gene | Petunia hybrida (Petunia, PETHY)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)Promoter
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BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)Terminator
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BCH-GENE-SCBD-103773-1 mac-1 PromoterPromoter
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BCH-GENE-SCBD-103774-1 Mannopine synthase gene terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
EN
EN
- Ornamental
- FLO-4Ø644-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
EN
These carnations were developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing two genes from petunia (Petunia hybrida) that function together in the biosynthesis of the anthocyanin pigment delphinidin. The transgenic lines were derived from the parent cultivar ‘White Unesco’, which is a white coloured carnation that was selected for a mutation in the dihydroflavonol reductase (DFR) encoding gene that did not allow for expression of a functional enzyme, and thus did not produce the anthocyanin type pigments that give rise to blue and red coloured flowers. The two genes from Petunia hybrida introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3’, 5’-hydroxylase (F3’5’H), a member of the NADPH-Cytochrome P450 reductase family. Expression of the F3’5’H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.
Tolerance to sulfonyl urea herbicides was produced via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco (surB).
EN
Tolerance to sulfonyl urea herbicides was produced via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco (surB).
- OECD UID Database [ English ]
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