MON-941ØØ-2 - Dicamba-tolerant canola | BCH-LMO-SCBD-258887 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 13 Jan 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Dicamba-tolerant canola
The canola (Brassica napus) was modified for tolerance to Dicamba (2-methoxy-3,6-dichlorobenzoic acid) by expressing Stenotrophomonas maltophilia dicamba monooxygenase, which demethylates the herbicide Dicamba to the non-herbicidal compounds 3,6-dicholorosalicylic acid (DCSA) and formaldehyde.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The modified canola (Brassica napus) contains one gene cassette: Stenotrophomonas maltophilia dicamba monooxygenase (dmo). The dmo coding sequence is under control of a Peanut chlorotic streak virus promoter and a Medicago truncatula hypothetical protein 3' untranslated region. In addition to the protein coding sequence, a Tobacco etch virus leader sequence (enhancing gene expression) and a Pisum sativum ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit transit peptide (directing the DMO protein to the chloroplast) are also present.

Next generation sequencing and directed sequencing indicated that the genetic elements in the insertion were intact and without rearrangements. Sequencing additionally indicated that a single T-DNA insertion was present in the parental genome and 8 basepairs of the parental genome were deleted during integration of the T-DNA.

The  PV-BNHT508701 vector contains two different T-DNA regions. Both T-DNA I (containing the genetic elements above) and T-DNA II were initially inserted into the canola genome. Subsequent crossing (via self-pollination) unlinked the two T-DNA insertions and allowed for the removal of T-DNA II from the parental genome. The genetic elements contained in T-DNA II are: Right border; Figwort mosaic virus enhancer; Arabidopsis thaliana elongation factor EF-1α; A. thaliana chloroplast transit peptide from ShkGaadA; P. sativum E9 terminator;  Vicia faba unknown seed protein promoter; A. tumefaciens splA; A. tumefaciens nopaline synthase terminator; and Left border. 

LMO characteristics
  • Feed
  • Food
Detection method(s)
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1