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Living Modified Organism (LMO)
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Dicamba-tolerant canola
EN
MON94100
MON-941ØØ-2
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PersonBayer CropScienceBayer CropScience AG Alfred-Nobel-Str. 50 40789 Monheim am RheinMonheim am Rhein,
40789, GermanyPhone: +49 21 73 - 38-0,Fax:Email:Related OrganizationBayer CropScience Deutschland GmbH ()Private sector (business and industry)Bayer CropScience AG Alfred-Nobel-Str. 50 40789 Monheim am RheinMonheim am Rhein,
40789, GermanyPhone: +49 21 73 - 38-0,Fax:Email:
The canola (Brassica napus) was modified for tolerance to Dicamba (2-methoxy-3,6-dichlorobenzoic acid) by expressing Stenotrophomonas maltophilia dicamba monooxygenase, which demethylates the herbicide Dicamba to the non-herbicidal compounds 3,6-dicholorosalicylic acid (DCSA) and formaldehyde.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12083-7 Organism Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Crops
PV-BNHT508701
EN
- Agrobacterium-mediated DNA transfer
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0.070 kb
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0.433 kb
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0.132 kb
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0.243 kb
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1.023 kb
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0.500 kb
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0.281 kb
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Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeatPlasmid vector
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeatPlasmid vector
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BCH-GENE-SCBD-115762-1 Leader sequence - Tobacco etch virus - TEV
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BCH-GENE-SCBD-104662-2 PC1SV Promoter | (PCSV, PClSV)Promoter
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BCH-GENE-SCBD-100728-2 Dicamba monooxygenase geneProtein coding sequence | Resistance to herbicides
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BCH-GENE-SCBD-258886-1 Hypothetical protein 3' untranslated region | Medicago truncatula (Barrelclover, Strong-spined medick, Barrel medic, Barrel medick, MEDTR)Terminator
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BCH-GENE-SCBD-103616-4 rbcS Transit Peptide | (garden pea)Transit signal
The modified canola (Brassica napus) contains one gene cassette: Stenotrophomonas maltophilia dicamba monooxygenase (dmo). The dmo coding sequence is under control of a Peanut chlorotic streak virus promoter and a Medicago truncatula hypothetical protein 3' untranslated region. In addition to the protein coding sequence, a Tobacco etch virus leader sequence (enhancing gene expression) and a Pisum sativum ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit transit peptide (directing the DMO protein to the chloroplast) are also present.
Next generation sequencing and directed sequencing indicated that the genetic elements in the insertion were intact and without rearrangements. Sequencing additionally indicated that a single T-DNA insertion was present in the parental genome and 8 basepairs of the parental genome were deleted during integration of the T-DNA.
Note:
The PV-BNHT508701 vector contains two different T-DNA regions. Both T-DNA I (containing the genetic elements above) and T-DNA II were initially inserted into the canola genome. Subsequent crossing (via self-pollination) unlinked the two T-DNA insertions and allowed for the removal of T-DNA II from the parental genome. The genetic elements contained in T-DNA II are: Right border; Figwort mosaic virus enhancer; Arabidopsis thaliana elongation factor EF-1α; A. thaliana chloroplast transit peptide from ShkG; aadA; P. sativum E9 terminator; Vicia faba unknown seed protein promoter; A. tumefaciens splA; A. tumefaciens nopaline synthase terminator; and Left border.
Next generation sequencing and directed sequencing indicated that the genetic elements in the insertion were intact and without rearrangements. Sequencing additionally indicated that a single T-DNA insertion was present in the parental genome and 8 basepairs of the parental genome were deleted during integration of the T-DNA.
Note:
The PV-BNHT508701 vector contains two different T-DNA regions. Both T-DNA I (containing the genetic elements above) and T-DNA II were initially inserted into the canola genome. Subsequent crossing (via self-pollination) unlinked the two T-DNA insertions and allowed for the removal of T-DNA II from the parental genome. The genetic elements contained in T-DNA II are: Right border; Figwort mosaic virus enhancer; Arabidopsis thaliana elongation factor EF-1α; A. thaliana chloroplast transit peptide from ShkG; aadA; P. sativum E9 terminator; Vicia faba unknown seed protein promoter; A. tumefaciens splA; A. tumefaciens nopaline synthase terminator; and Left border.
- Feed
- Food
- EUginius - MON94100 oilseed rape ( English )
- A1216 -Application to Food Standards Australia New Zealand for the Inclusion of Canola MON 94100 in Standard 1.5.2 - Food Derived from Gene Technology.pdf ( English )
- CA3057917A1 - Brassica event mon94100 and methods of use thereof.pdf ( English )
- CA3057917A1 - Evenement mon94100 brassica et procedes d`utilisation associes ( French )
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