CTC-75Ø64-3 - Insect-resistant sugarcane | BCH-LMO-SCBD-261453 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 25 Aug 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant sugarcane
The sugarcane (Saccharum officinarum) was transformed using Agrobacterium-mediated transformation for resistance to cane borer (Diatraea saccharalis) through the expression of Bacillus thuringiensis cry1Ac. The sugarcane additionally contains an Escherichia coli neomycin phosphotransferase II cassette, which allowed for kanamycin selection during transformation. The genetic background of CTC75064-3 is cultivar RB867515.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety RB867515
  • CTC-95Ø19-5 - Insect-resistant sugarcane
    | Dr Wladecir Salles Oliveira | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Selectable marker genes and reporter genes
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The modified sugarcane contains two gene cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase (nptII).

The cry1Ac coding sequence is under control of the a Cauliflower mosaic virus (CaMV) 35S promoter (with a duplicated enhancer region) and a CaMV 35 terminator. Additionally, an 5' untranslated leader from chlorophyll a/b-binding leader sequence and Oryza sativa actin intron 1 were added to enhance gene expression. Due to the additional untranslated sequences and the enhanced promoter, high levels of transcription are expected to occur in all plant tissues.

The nptII coding sequence in under control of a Zea mays ubiquitin 1 promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected to occur in all plant tissues.

  • The DNA integrated into the genome of event CTC75064-3 was characterized using several methodologies. The number of copies of heterologous genes was previously estimated by means of quantitative real-time PCR (qPCR). The results indicated that the sugarcane genome contains a single copy of the insert, as well as a single copy of both cry1Ac and nptII genes.  No integration of vector backbone sequences were detected. Southern blot analysis using probes homologous to the cry1Ac gene and nptII gene sequences supported the qPCR results and also indicated the absence of partial integration of T-DNA elements at other sites in the genome of the CTC75064-3 event. The degree of genotypic stability of the event CTC75064-3 was also verified via Southern blot methodology, which proved that the T-DNA insert remained stable over four generations of vegetative propagation representing the different crop cycles (plant-cane and ratoon-cane).
LMO characteristics
  • Biofuel
  • Feed
  • Food
Detection method(s)
See attached patent documents.