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Living Modified Organism (LMO)
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Insect-resistant sugarcane
EN
CTC75064-3
Yes
CTC-75Ø64-3
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Organization:CTC - Centro de Tecnologia Canavieira (CTC)Regional economic integration organizationFazenda Santo Antônio s/n, Caixa Postal 162Piracicaba, Sao Paulo
13400-970, BrazilPhone: +55 (11) 3429-8111,Fax: +55 (11) 3429-8111,Email: Wladecir.oliveira@ctc.com.br,Website: http://new.ctc.com.br/,
The sugarcane (Saccharum officinarum) was transformed using Agrobacterium-mediated transformation for resistance to cane borer (Diatraea saccharalis) through the expression of Bacillus thuringiensis cry1Ac. The sugarcane additionally contains an Escherichia coli neomycin phosphotransferase II cassette, which allowed for kanamycin selection during transformation. The genetic background of CTC75064-3 is cultivar RB867515.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-115592-1 Organism Saccharum officinarum L. (Sugarcane, Sugar cane)Crops
Variety RB867515
EN
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CTC-95Ø19-5 - Insect-resistant sugarcane| Dr Wladecir Salles Oliveira | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Selectable marker genes and reporter genes
pCTC523
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-100354-6 5' untranslated leader from chlorophyll a/b-binding protein | Triticum aestivum (Wheat)Leader sequence
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
The modified sugarcane contains two gene cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase (nptII).
The cry1Ac coding sequence is under control of the a Cauliflower mosaic virus (CaMV) 35S promoter (with a duplicated enhancer region) and a CaMV 35 terminator. Additionally, an 5' untranslated leader from chlorophyll a/b-binding leader sequence and Oryza sativa actin intron 1 were added to enhance gene expression. Due to the additional untranslated sequences and the enhanced promoter, high levels of transcription are expected to occur in all plant tissues.
The nptII coding sequence in under control of a Zea mays ubiquitin 1 promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected to occur in all plant tissues.
Note:
The cry1Ac coding sequence is under control of the a Cauliflower mosaic virus (CaMV) 35S promoter (with a duplicated enhancer region) and a CaMV 35 terminator. Additionally, an 5' untranslated leader from chlorophyll a/b-binding leader sequence and Oryza sativa actin intron 1 were added to enhance gene expression. Due to the additional untranslated sequences and the enhanced promoter, high levels of transcription are expected to occur in all plant tissues.
The nptII coding sequence in under control of a Zea mays ubiquitin 1 promoter and Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, high levels of expression are expected to occur in all plant tissues.
Note:
- The DNA integrated into the genome of event CTC75064-3 was characterized using several methodologies. The number of copies of heterologous genes was previously estimated by means of quantitative real-time PCR (qPCR). The results indicated that the sugarcane genome contains a single copy of the insert, as well as a single copy of both cry1Ac and nptII genes. No integration of vector backbone sequences were detected. Southern blot analysis using probes homologous to the cry1Ac gene and nptII gene sequences supported the qPCR results and also indicated the absence of partial integration of T-DNA elements at other sites in the genome of the CTC75064-3 event. The degree of genotypic stability of the event CTC75064-3 was also verified via Southern blot methodology, which proved that the T-DNA insert remained stable over four generations of vegetative propagation representing the different crop cycles (plant-cane and ratoon-cane).
EN
- Biofuel
- Feed
- Food
See attached patent documents.
EN
- EP3995583A1- Polynucleotides, primers, and methods for detection of transgenic event, genetic construct, kit for detection material from a plant sample, event ctc75064-3, insect-resistant sugarcane plant, and method for producing an insect-resistant sugarcane plant, plant cell, plant part or seed [ English ]
- EP3995583A1 - event ctc75064-3.pdf [ English ]
- CropLife Brazil - CTC-75064-3 [ Portuguese ]
