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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant eucalyptus
EN
955S019
Yes
FGN-Ø3S19-5
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,Organization:FuturaGene Brasil Tecnologia Ltda ()Private sector (business and industry)
, BrazilPhone: + 55 15 3511 8032,Fax:Email: info@futuragene.com,Website: -
Organization:Suzano S.A. ()Escritório São Paulo Avenida Brigadeiro Faria Lima, 1355São Paulo, São Paulo
, BrazilPhone: +55 800 022 1727, +55 11 3503 9000,Fax:Email: suzanoresponde@suzano.com.br,Website: https://www.suzano.com.br/,
Eucalyptus (Eucalyptus urophylla) was modified for tolerance to herbicides (for weed management). For tolerance to glyphosate, the modified eucalyptus expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which does not bind the herbicide with high affinity. Thus, the transgene allows for the continued functioning of the shikimate pathway and thus the biosynthesis of aromatic amino acids and compounds. In addition to herbicide tolerance gene cassettes, an Escherichia coli neomycin phosphotransferase II cassette was included as a selectable marker during the transformation of the eucalyptus line.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-272517-3 Organism Eucalyptus urophylla (Timor mountain gum, Timor white gum, Eucalyptus, EUCUR)Trees
Variety FGN-S
EN
pBI121
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-115762-1 Leader sequence | Tobacco etch virus (TEV)Leader sequence
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
The modified eucalyptus expresses three gene cassettes: two Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) cassettes and one Escherichia coli neomycin phosphotransferase II (nptII) cassette.
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
- Molecular characterization indicated that a single T-DNA insertion occurred in the eucalyptus genome (in chromosome 3) without inclusion of vector backbone sequences. The sequences are present in the same arrangement as the transformation vector (i.e., without rearrangement). Protein expression was confirmed with ELISA.
EN
- Timber
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Recipient Organism or Parental Organisms | 3 | |
