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Central and Eastern European Workshop on the Detection and Identification of LMOs

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Opening of Discussion: Extraction Procedures [#7933]
POSTED ON BEHALF OF MOJCA MILAVEC
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Dear Participants of the Forum,

I am pleased to welcome you to the second round of online discussions of CEE workshop on the detection and identification of LMOs.

As agreed at the workshop, and outlined in the report of the meeting (which is available at http://www.cbd.int/doc/meetings/bs/bsdiws-2016-01/official/bsdiws-2016-01-02-en.pdf), the second round of the online discussions is dedicated to the extraction mathods. The aim of this discussion is compilation of information and best practices on the most efficient extraction procedures for specific matrices and considerations for troubleshooting difficult samples.
I would like to invite you to post any relevant material on the matter, especially your experiences with different matrices and extraction and purification methods you are using as well as pose questions and comments on specific areas where additional information is needed so that others that may have this information available can share it with the group.

The discussion will take place for the next two weeks. Your views and feedback will be greatly appreciated. If possible, please post your comments during the early days of the discussion to foster a more lively debate building on the comments of one another.

I look forward to your comments and suggestions!
Kindest regards,
Mojca
posted on 2016-05-30 01:46 UTC by Dina Abdelhakim, SCBD
RE: Opening of Discussion: Extraction Procedures [#7951]
Dear Forum Participants,
I would like to remind you that the forum on the "Compilation of information and best practices on extraction procedures" is still open.
I urge you to post some resources, questions or comments on this topic with the view to create the tables as requested in the workshop.
I look forward to reading your interventions,
Kind regards,
Dina
posted on 2016-06-07 18:20 UTC by Dina Abdelhakim, SCBD
RE: Opening of Discussion: Extraction Procedures [#7954]
Dear Forum Participants,
I would like to share with you experiences from our lab with different sample types (matrices) and methods used for thier homogenisation and extraction. I invite you to look at the table and share your experiences or post comments or quantions.

Kind regards,

Mojca
posted on 2016-06-09 05:32 UTC by dr Mojca Milavec, Slovenia
RE: Opening of Discussion: Extraction Procedures [#7970]
Hallo,
I was not in the lab last two weeks
Thank you Mojca for the table
I think it will be nice if you have enough data to add one more column to that table with expected amount of DNA
One more think- if you " modified" some of extraction methods for the specific matrices- Would you share?
Thank you in advance
Krasimira
(edited on 2016-06-15 06:32 UTC by Krasimira Ivanova, Executive Environment Agency)
posted on 2016-06-15 06:30 UTC by Krasimira Ivanova, Executive Environment Agency