Gene expression
- The genetic cassettes are present in the clockwise (sense) orientation.
- Transcription of all of the coding sequence, except  Arabidopsis thaliana acetohydroxy acid synthase large-subunit (Atahas), are under control of seed-specific promoters.
- Atahas is under control of Petroselinum crispum ubiquitin 4-2 promoter, which promotes strong and constitutive expression in all vegetative tissues.
- Some gene cassetes contain introns to enhance the expression of the coding sequence.

Inserted elements
- Agrobacterium rhizogenes-mediated transformation was used instead of the common Agrobacterium tumefaciens-mediated transformation.
- Conlinin promoter was not specified (whether Conlinin-1 or -2). Thus, Conlinin-1 promoter was chosen as a placeholder.
- Two Thraustochytrium sp. delta-6 desaturase and two P. irregulare omega-3 desaturase sequences are present per insert.
- Arabidopsis thaliana acetohydroxy acid synthase large-subunit contains S653N and A122T substitutions.
- The following coding sequences were codon optimized for expression in plants:
   -- Thraustochytrium sp. delta-5 desaturase;
   -- Ostreococcus tauri delta-6 desaturase;
   -- Thalassiosira pseudonana delta-6 elongase;
   -- Phytophthora sojae delta-12 desaturase;
   -- Pythium irregulare omega-3 desaturase;
   -- Phytophthora infestans omega-3 desaturase;
   -- Thraustochytrium sp. delta-4 desaturase;
   -- Pavlova lutheri delta-4 desaturase; and
   -- Ostreococcus tauri delta-5 elongase.

Vector backbone
- The backbone contains: Escherichia coli neomycin phosphotransferase II; E. coli origin of replication (F plasmid); E. coli sopA and sopB; Agrobacterium tumefaciens repB and repC; E. coli Tn5 sequence; and A. tunmefaciens oriT.

Genomic analysis by next generation sequencing (Illumina)
- Next generation sequencing confirmed two separate T-DNA insertions are present, without any rearrangement of the genetic cassettes.
- Insert 1 contained a 184-basepair truncation of the 5΄ end of the RB and a 72-basepair truncation of the 3΄ end of the LB. The first 64 basepairs in the RB of Insert 1 was determined to be a rearrangement of short T-DNA RB-derived repeats. Insert 2 contained 184-basepair truncation of the 5΄ end of the RB with a 53-basepair truncation of the 3΄ end of the LB.
- Insert 1 and Insert 2 contained all 13 intended gene expression cassettes.
- Insert 1 had one cytosine to adenine nucleotide change in the coding sequence of the delta-12 desaturase gene, resulting in a phenylalanine to leucine amino acid substitution (F83L) in the protein, and a nucleotide change in the L. usitatissimum peroxiredoxin-like protein promoter of the P. irregulare omega-3 desaturase.
- Insert 1 was inserted into the the Cnn-random chromosome and resulted in a 8-basepair deletion in the canola genome.
- Insert 2 contains a guanine to thymine nucleotide change in the coding sequences of P. lutheri delta-4 desaturase, resulting in an alanine to serine amino acid substitution (A102S) in the protein.
- Insert 2 was inserted into the C03 chromosome and resulted in a 31-basepair deletion in the canola genome.
- The amino acid changes in the proteins did not show altered activity.
- No backbone sequences were detected.