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Living Modified Organism (LMO)
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Cassava modified for increased levels of iron and zinc
EN
D8023-985004
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OrganizationNational Root Crops Research Institute (NRCRI)Academic or research instituteKm 8 Ikot Ekpene Road, PMB 7006Umuahia, Abia
, NigeriaPhone: +234 (0)8168983790, +2349035068714,Fax:Website: https://nrcri.gov.ng/,
Cassava (Manihot esculenta Crantz) was modified to express Arabidopsis thaliana FERRITIN 1 and IRON-REGULATED TRANSPORTER 1 to increase the levels of iron and zinc in the tuber tissues. Previous overexpression experiments demonstrated increases of iron by 7-18 times and zinc by 3-10 times.
A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-112539-1 Organism Manihot esculenta (Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES)Crops
Cassava cultivar: TMS 98/0505
p8023
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated
forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-115111-1 Phloem protein 2-A14 promoter | (Thale cress)Promoter
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BCH-GENE-SCBD-115112-1 Iron-regulated transporter 1 | (Thale cress)Protein coding sequence | Changes in quality and/or metabolite content,Increased levels of iron
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BCH-GENE-SCBD-115113-1 Phloem protein 2-A14 3' untranslated region | (Thale cress)Terminator
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BCH-GENE-SCBD-100290-6 CaMV 35S terminatorTerminator
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BCH-GENE-SCBD-100273-4 B33 gene promotor | (Potato)Promoter
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BCH-GENE-SCBD-115136-1 Ferritin 1 | (Thale cress)Protein coding sequence | Changes in quality and/or metabolite content,Increased levels of iron,Tolerance to abiotic stress,Tolerance to excess iron
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BCH-GENE-SCBD-115137-1 Patatin-1 3' untranslated region | (Potato)Terminator
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene TerminatorTerminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | (Bacteria)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoterPromoter
Iron-regulated transporter cassette:
Transcription of the Arabidopsis thaliana iron-regulated transporter 1 is under control of the A. thaliana phloem protein 2-A14 promoter and 3' untranslated region, as well as the Cauliflower Mosaic Virus 35S terminator.
Ferritin-1 cassette:
Arabidopsis thaliana ferritin-1 is under transcriptional control of the Solanum tuberosum patatin-1 promoter and terminator, as well as the Agrobacterium tumefaciens nopaline synthase (nos) terminator.
Selectable marker cassette:
Transcription of the Escherichia coli neomycin phosphotransferase II occurs from the duplicated enhanced Cauliflower Mosaic Virus 35S promoter and terminates at the nos terminator.
Please note all genetic elements were in the anti-sense orientation.
Transcription of the Arabidopsis thaliana iron-regulated transporter 1 is under control of the A. thaliana phloem protein 2-A14 promoter and 3' untranslated region, as well as the Cauliflower Mosaic Virus 35S terminator.
Ferritin-1 cassette:
Arabidopsis thaliana ferritin-1 is under transcriptional control of the Solanum tuberosum patatin-1 promoter and terminator, as well as the Agrobacterium tumefaciens nopaline synthase (nos) terminator.
Selectable marker cassette:
Transcription of the Escherichia coli neomycin phosphotransferase II occurs from the duplicated enhanced Cauliflower Mosaic Virus 35S promoter and terminates at the nos terminator.
Please note all genetic elements were in the anti-sense orientation.
- Food
- Research
Please note the following:
- This line was developed in parallel to another line with the same genetics. The only difference is that the other recipient cultivar was TMS 91/02324.
- The transformation event state is a placeholder. Several lines were developed and are expected to have similar genetics: D8023-985004, D8023-985005, D8023-985006, D8023-985009, D8023-985010, D8023-985011, and D8023-985012.
- The transformation event will be updated when more information is available and/or a line has been chosen for commercialization.
- This line was developed in parallel to another line with the same genetics. The only difference is that the other recipient cultivar was TMS 91/02324.
- The transformation event state is a placeholder. Several lines were developed and are expected to have similar genetics: D8023-985004, D8023-985005, D8023-985006, D8023-985009, D8023-985010, D8023-985011, and D8023-985012.
- The transformation event will be updated when more information is available and/or a line has been chosen for commercialization.
- Biofortification of field-grown cassava.pdf [ English ]
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