Cassava modified for increased levels of iron and zinc | BCH-LMO-SCBD-115141 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 23 Aug 2019
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Cassava modified for increased levels of iron and zinc
EN
D8023-985004
Cassava (Manihot esculenta Crantz) was modified to express Arabidopsis thaliana FERRITIN 1 and IRON-REGULATED TRANSPORTER 1 to increase the levels of iron and zinc in the tuber tissues. Previous overexpression experiments demonstrated increases of iron by 7-18 times and zinc by 3-10 times.

A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cassava cultivar: TMS 98/0505
EN
  • Cassava modified for increased levels of iron and zinc
    | National Root Crops Research Institute(NRCRI) | Changes in quality and/or metabolite content, Increased levels of iron, Increased levels of zinc, Resistance to antibiotics (Kanamycin), Selectable marker genes and reporter genes
  • Viral resistant cassava with increased levels of zinc and iron
    | National Root Crops Research Institute(NRCRI) | Changes in quality and/or metabolite content, Increased iron levels, Increased zinc levels, Resistance to antibiotics (Kanamycin), Resistance to CBSV, Resistance to diseases and pests (Viruses), Resistance to UCBSV, Selectable marker genes and reporter genes
  • Viral resistant cassava with increased levels of zinc and iron
    | Dr Ihuoma Okwuonu | Changes in quality and/or metabolite content, Increased iron levels, Increased zinc levels, Resistance to antibiotics (Kanamycin), Resistance to CBSV, Resistance to diseases and pests (Viruses), Resistance to UCBSV, Selectable marker genes and reporter genes
Characteristics of the modification process
p8023
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Iron-regulated transporter cassette:
Transcription of the Arabidopsis thaliana iron-regulated transporter 1 is under control of the A. thaliana phloem protein 2-A14 promoter and 3' untranslated region, as well as the Cauliflower Mosaic Virus 35S terminator.

Ferritin-1 cassette:
Arabidopsis thaliana ferritin-1 is under transcriptional control of the Solanum tuberosum patatin-1 promoter and terminator, as well as the Agrobacterium tumefaciens nopaline synthase (nos) terminator.

Selectable marker cassette:
Transcription of the Escherichia coli neomycin phosphotransferase II occurs from the duplicated enhanced Cauliflower Mosaic Virus 35S promoter and terminates at the nos terminator.

Please note all genetic elements were in the anti-sense orientation.
EN
LMO characteristics
EN
  • Food
  • Research
Detection method(s)
EN
Additional Information
Please note the following:
- This line was developed in parallel to another line with the same genetics. The only difference is that the other recipient cultivar was TMS 91/02324.
- The transformation event state is a placeholder. Several lines were developed and are expected to have similar genetics: D8023-985004, D8023-985005, D8023-985006, D8023-985009, D8023-985010, D8023-985011, and D8023-985012.
- The transformation event will be updated when more information is available and/or a line has been chosen for commercialization.
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Record type Field Record(s)