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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
TELA® Maize
EN
MON 87460 × MON 89034
Yes
MON-8746Ø-4 × MON-89Ø34-3
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Person:Bayer Crop Science CompanyP.O. Box 79345-00200 KenyaNairobi,
, KenyaPhone: +25478 6666087,Fax:Email: simonevans.njeru@bayer.com,Website:Related OrganizationBayer Crop Science Company ()Private sector (business and industry)P.O. Box 79345-00200 KenyaNairobi,
, KenyaPhone: +25478 6666087,Fax:Email: simonevans.njeru@bayer.com,Website: -
Person:AATF and Institute of Agricultural ResearchARCN Annex No. 3 Ibrahim Idris Street, Jabi( AATF) Institute of Agricultural Research, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria.Abuja, F.C.T
, NigeriaPhone: 254-204223700, +234 8028373464,Fax: 254-204223701,Email: rsadamu@gmail.com,Website:Related OrganizationAfrican Agricultural Technology Foundation and Institute of Agricultural Research ()Academic or research instituteARCN Annex No. 3 Ibrahim Idris Street, Jabi( AATF) Institute of Agricultural Research, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria.Abuja, F.C.T
, NigeriaPhone: 254-204223700, +234 8028373464,Fax: 254-204223701,Email: rsadamu@gmail.com,Website:
The drought-tolerant, insect-resistant maize (MON 87460 × MON 89034) was obtained through crossing the two maize event products: MON 87460 and MON 89034. The modified maize expresses Bacillus subtilis cold shock protein (from MON97460), which confers cold and drought tolerance by enhancing natural abiotic stress responses. The maize also expresses the Bacillus thuringiensis insecticidal proteins Cry1A.105 and Cry2Ab2 (from MON 89034), which confer resistance to Lepidoptera pests (particularly fall armyworm and stem borer). Additionally, a selectable marker for kanamycin resistance (Escherichia coli neomycin phosphotransferase II) is expected to be present as it was used for selection of transformants during the generation of the parental MON 87460 line.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-LMO-SCBD-103066-6 Living Modified Organism MON-8746Ø-4 - Droughtgard™ MaizeResistance to antibiotics (Kanamycin, Neomycin), Tolerance to abiotic stress (Cold / Heat, Drought)
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BCH-LMO-SCBD-43773-18 Living Modified Organism MON-89Ø34-3 - YieldGard™ VT Pro™Monsanto Company | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
EN
PV-ZMAP595; PV-ZMIR245
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-103065-7 Cold shock protein gene | Bacillus subtilis (Bacillus, BACIU)Protein coding sequence | Tolerance to abiotic stress (Cold / Heat, Drought)
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103069-3 loxP recombination site | Bacteriophage P1 (Phage P1)recombination site
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100354-6 5' untranslated leader from chlorophyll a/b-binding protein | Triticum aestivum (Wheat)Leader sequence
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BCH-GENE-SCBD-43771-9 Cry1A.105 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100356-6 Heat shock protein 17.3 terminator | Triticum aestivum (Wheat)Terminator
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100360-4 Transit peptide and first intron of Rubisco SSU | Zea mays (Maize, Corn, MAIZE)Transit signal
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BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
Genetic elements introduced from PV-ZMAP595
Two gene cassettes were integrated from this vector.
I. Transcription of Bacillus subtilis cold shock protein (cspB) begins from the Oryza sativa (rice) actin 1 promoter and ends at the Agrobacterium tumefaciens 3' untranslated region of transcript 7. Transcript contains the rice actin 1 intron at the 5' end. The intron is expected to enhance gene expression of cspB.
II. Transcription of the Escherichia coli neomycin phosphotransferase II (nptII) is under the control of the Cauliflower mosaic virus (CaMV) 35S promoter and the A. tumefaciens nopaline synthase terminator (nos). The gene cassette is flanked by Bacteriophage P1 locus of cross-over P1 (loxP) sites.
Please note
- The parental line contains a single insertion of the T-DNA from this vector.
- No vector backbone sequence was detected.
- The parental line contains intact genetic cassettes.
Genetic elements introduced from PV-ZMIR245
Two gene cassettes were present in the parental line.
III. Transcription of the Bacillus thuringiensis crystal 1A.105 (cry1A.105) commences from the CaMV enhanced 35S promoter and terminates at the Triticum aestivum (wheat) heat shock protein 17.3 terminator. The transcript contains a rice actin 1 intron and a wheat chlorophyll a/b-binding protein 5' leader for enhanced gene expression.
IV. Transcription of B. thuringiensis cry2Ab2 is under the control of the Figwort Mosaic Virus 35S promoter and the A. tumefaciens nos terminator. Zea mays heat shock protein 70 intron and transit peptide form Rubisco small subunit are also present in the transcript at the 5' end for enhanced gene expression and chloroplast targeting, respectively.
Please note
- The cry2Ab2 coding sequence was optimized for expression in plants.
- An additional nptII cassette in reverse orientation was present in the pV-ZMIR245 vector and inserted as a secondary, unlinked T-DNA. During the development of the parental line, selective breeding was done to remove the nptII marker, resulting in a marker-free parental line.
- Southern blot analysis confirmed a single insertion and expression of the other T-DNA containing the genetic cassettes mentioned above (III and IV).
- DNA sequencing indicated that enhanced CaMV promoter did not contain the duplicated enhancer regions.
- No vector backbone was detected in the parental line.
For more information, kindly refer to the parental modified organism records
EN
Two gene cassettes were integrated from this vector.
I. Transcription of Bacillus subtilis cold shock protein (cspB) begins from the Oryza sativa (rice) actin 1 promoter and ends at the Agrobacterium tumefaciens 3' untranslated region of transcript 7. Transcript contains the rice actin 1 intron at the 5' end. The intron is expected to enhance gene expression of cspB.
II. Transcription of the Escherichia coli neomycin phosphotransferase II (nptII) is under the control of the Cauliflower mosaic virus (CaMV) 35S promoter and the A. tumefaciens nopaline synthase terminator (nos). The gene cassette is flanked by Bacteriophage P1 locus of cross-over P1 (loxP) sites.
Please note
- The parental line contains a single insertion of the T-DNA from this vector.
- No vector backbone sequence was detected.
- The parental line contains intact genetic cassettes.
Genetic elements introduced from PV-ZMIR245
Two gene cassettes were present in the parental line.
III. Transcription of the Bacillus thuringiensis crystal 1A.105 (cry1A.105) commences from the CaMV enhanced 35S promoter and terminates at the Triticum aestivum (wheat) heat shock protein 17.3 terminator. The transcript contains a rice actin 1 intron and a wheat chlorophyll a/b-binding protein 5' leader for enhanced gene expression.
IV. Transcription of B. thuringiensis cry2Ab2 is under the control of the Figwort Mosaic Virus 35S promoter and the A. tumefaciens nos terminator. Zea mays heat shock protein 70 intron and transit peptide form Rubisco small subunit are also present in the transcript at the 5' end for enhanced gene expression and chloroplast targeting, respectively.
Please note
- The cry2Ab2 coding sequence was optimized for expression in plants.
- An additional nptII cassette in reverse orientation was present in the pV-ZMIR245 vector and inserted as a secondary, unlinked T-DNA. During the development of the parental line, selective breeding was done to remove the nptII marker, resulting in a marker-free parental line.
- Southern blot analysis confirmed a single insertion and expression of the other T-DNA containing the genetic cassettes mentioned above (III and IV).
- DNA sequencing indicated that enhanced CaMV promoter did not contain the duplicated enhancer regions.
- No vector backbone was detected in the parental line.
For more information, kindly refer to the parental modified organism records
EN
- Food
- Feed
- MON-89Ø34-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-8746Ø-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- GMO Detection Method Database - MON87460 [ English ]
- GMO Detection Method Database - MON89034 [ English ]
- MON-8746Ø-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-89Ø34-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-8746Ø-4 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-89Ø34-3 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
EN
- EUginius - MON87460 x MON89034 [ English ]
- AATF - TELA Maize project [ English ]
- CIMMYT - TELA Maize project [ English ]
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