FLO-4Ø619-8 - Moonshade™ carnation | BCH-LMO-SCBD-14828 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 23 Nov 2020
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moonshade™ carnation
EN
123.2.2 (40619)
Yes
FLO-4Ø619-8
Carnations with modified colour and sulfonylurea herbicide tolerance, produced by inserting two anthocyanin biosynthetic genes from Petunia hybrida, dihydroflavonol reductase (dfr) and Hf1 encoding flavonoid 3',5'hydroxylase (F3'5'H), whose expression results in a violet/mauve colouration. Tolerance to sulfonyl urea herbicides was produced through the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from Nicotiana tabacum.

NOTE: This LMO was formerly referred to with the UID FLO-4Ø619-7.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
A white carnation cultivar FE 123, belonging to Dianthus caryophyllus L., Dianthus, Caryophyllaceae, was used as the recipient organism.
EN
  • BCH-LMO-SCBD-14836-7
    FLO-Ø7442-5 - Moondust™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • BCH-LMO-SCBD-14834-6
    FLO-4Ø644-6 - Moonlite™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • BCH-LMO-SCBD-14837-7
    FLO-ØØØ15-3 - Moondust™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • BCH-LMO-SCBD-14838-7
    FLO-ØØØ16-4 - Moondust™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • BCH-LMO-SCBD-14840-7
    FLO-ØØØØ4-1 - Moondust™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Sulfonylurea)
  • BCH-LMO-SCBD-115777-1
    FLO-4Ø62Ø-9 - Moonburst™ carnation
    | SUntory Holdings Ltd. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
Characteristics of the modification process
pCGP1470
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15009-4 Dihydroflavonol-4-reductase | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-15010-3 Flavonoid 3', 5' hydroxylase gene | Petunia hybrida (Petunia, PETHY)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )
    Terminator
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
    Promoter
  • BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
    Terminator
  • BCH-GENE-SCBD-103773-1 mac-1 Promoter
    Promoter
  • BCH-GENE-SCBD-103774-1 Mannopine synthase gene terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-45875-7 Beta-galactosidase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
    Leader
Gene expression
The T-DNA insertion contained three gene cassettes: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida flavonoid 3', 5' hydroxylase (F3'5'H) and P. hybrida dihydroflavonol-4-reductase (DFR).

Transcription of ALS is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and the ALS terminator. A 5' untranslated leader from P. hybrida chlorophyll a/b-binding protein is also transcribed, but is not expected to be translated. The leader sequence augments the level of transcription (enhances gene expression) of ALS.

Transcription of F3'5'H is under control of the Antirrhinum majus chalcone synthase gene promoter and the P. hybrida D8 terminator.

Transcription of DFR is under control of the synthetic Mac-1 promoter and the Agrobacterium tumefaciens mannopine synthase gene terminator.


Note:
- The genetic element size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of coding sequence + terminator)
- The Mac-1 promoter is a synthetic promoter compromised of A. tumefaciens mas promoter and CaMV enhancer sequences.
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
Additional Information
This transgenic carnation was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing two genes from petunia (Petunia hybrida) that function together in the biosynthesis of the anthocyanin pigment delphinidin. The transgenic lines were derived from the parent cultivar ‘White Unesco’, which is a white coloured carnation that was selected for a mutation in the dihydroflavonol reductase (DFR) encoding gene that did not allow for expression of a functional enzyme, and thus did not produce the anthocyanin type pigments that give rise to blue and red coloured flowers. The two genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene and a gene encoding the enzyme flavonoid 3’, 5’-hydroxylase (F3’, 5’H), a member of the NADPH-Cytochrome P450 reductase family. Expression of these genes allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 4
Country's Decision or any other Communication Living modified organism(s) 4
Living Modified Organism Recipient Organism” or “Parental Organisms 1
Living Modified Organism Related LMO(s) 5

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