ACS-GMØØ4-2 - Herbicide-tolerant soybean | BCH-LMO-SCBD-14855 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 18 Jan 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant soybean
The soybean line A2704-12 was developed to allow for the use of glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta® , Ignite® , Rely® , Liberty® , Harvest® , and Finale®) as a weed control option. These genetically engineered soybean lines contain the fungal enzyme phosphinothricin-N-acetyltransferase (PAT) that allows these plants to survive the otherwise lethal application of glufosinate. The pat gene inserted was isolated from a common soil fungus, Streptomyces viridochromogenes, and introduced into the soybean genome by particle acceleration (biolistic) transformation.

The PAT enzyme converts L-phosphinothricin (PPT), the active ingredient in glufosinate ammonium, to an inactive form thereby conferring resistance to the herbicide. In absence of PAT, application of glufosinate leads to reduced production of the amino acid glutamine and increased ammonia levels in the plant tissues which are lethal to the plant. The PAT enzyme is not known to have any toxic properties.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
pB2/35SAcK (AKA: pWRG5143)
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The PAT gene from Streptomyces viridochromogenes was synthetically modified with plant preferred codons.

The bacterial ampicillin resistance gene was also integrated into the host genome. It is however only expressed in bacterial cells.
LMO characteristics
  • Food
Detection method(s)
Southern blot analysis indicated that 5 copies of the PAT gene and 2 copies of the ampR gene were integrated into the host genome.
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 3
Living Modified Organism Related LMO(s) 6
Country's Decision or any other Communication Living modified organism(s) 2
Risk Assessment generated by a regulatory process Living modified organism(s) 1