BCS-GH811-4 × BCS-GHØØ4-7 × BCS-GHØØ5-8 × SYN-IR1Ø2-7 × MON-887Ø1-3 - Herbicide-tolerant, insect-resistant cotton | BCH-LMO-SCBD-262938 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 20 Jan 2023
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant cotton
EN
GHB811 × T304-40 × GHB119 × COT102 × MON88701
Yes
BCS-GH811-4 × BCS-GHØØ4-7 × BCS-GHØØ5-8 × SYN-IR1Ø2-7 × MON-887Ø1-3
  • - Person: Bayer CropScience Bayer CropScience | BCH-CON-US-7091-2
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    Bayer CropScience Bayer CropScience
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    Related Organization
    Bayer CropScience ()
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The modified cotton (Gossypium hirsutum) was produced by crossing modified parental varieties for insect resistance and multiple herbicide tolerance. For resistance to Lepidoptera pests, the cotton expresses Bacillus thuringiensis delta-endotoxins Cry1Ab and Cry12Ae, as well as vegetative insecticidal protein 3A. These proteins disrupt the midgut epithelial lining of feeding larvae, leading to death. To confer resistance to isoxaflutole, the cotton expresses Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD) W336, which contains a point mutation that confers reduced binding affinity to HPPD inhibitors. For tolerance to glyphosate, the cotton expresses the Zea mays double mutant 5-enol pyruvylshikimate-3-phosphate synthase, which has a reduced binding affinity to the herbicide.  For tolerance to dicamba, the cotton expresses Stenotrophomonas maltophilia dicamba monooxygenase, which inactivates the herbicide via oxidative demethylation. For tolerance to glufosinate, the cotton expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivate the herbicide via acetylation. In addition, Escherichia coli hygromycin-B phosphotransferase is also expressed. It was included as a selectable marker during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)
    Crops
  • BCH-LMO-SCBD-113966-4 Living Modified Organism BCS-GH811-4 - Herbicide-tolerant cotton
    Bayer CropScience | Resistance to herbicides (Glyphosate)
  • BCH-LMO-SCBD-14992-9 Living Modified Organism SYN-IR1Ø2-7 - VIPCOT™ Cotton
    Syngenta | Resistance to antibiotics (Hygromycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Selectable marker genes and reporter genes
  • BCH-LMO-SCBD-101018-14 Living Modified Organism BCS-GHØØ4-7 - Herbicide-tolerant, insect-resistant cotton
    Bayer Crop Science K.K | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-101898-6 Living Modified Organism BCS-GHØØ5-8 - Insect-protected, herbicide-tolerant cotton
    Bayer BioScience N.V. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), Fall armyworm (Spodoptera frugiperda)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-105602-3 Living Modified Organism MON-887Ø1-3 - Dicamba- and Glufosinate-tolerant cotton
    Monsanto | Resistance to herbicides (Glufosinate)
EN
Characteristics of the modification process
pTSIH09; pTDL008; pTEM12; pCOT-1; PV-GHHT6997
EN
  • Cross breeding
 
0.000 kb
 
 
0.940 kb
 
 
1.850 kb
 
 
0.060 kb
 
 
1.040 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-104647-3 Histone H4 gene Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-104648-2 Histone H3 Gene II intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-101419-4 Optimized Transit Peptide
    Transit signal
  • BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-104646-4 Histone H4 gene 3' UTR | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Terminator
  • BCH-GENE-SCBD-101900-6 CsVMV promoter | Cassava vein mosaic virus (Cassava vein mosaic virus, CVMV, CsVMV)
    Promoter
  • BCH-GENE-SCBD-104793-3 4-hydroxyphenylpyruvate dioxygenase | Pseudomonas fluorescens (PSEFL)
    Protein coding sequence | Resistance to herbicides
  • BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101025-5 NADP-malic enzyme 1 gene 3'UTR and terminator | Flaveria bidentis (Coastal plain yellowtops, FLABI)
    Terminator
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-104947-3 5'e1 Leader | Oryza sativa (Rice, ORYSA)
    Leader
  • BCH-GENE-SCBD-101021-3 Ps7s7 | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)
    Promoter
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
    Leader
  • BCH-GENE-SCBD-101902-4 rbcS Transit Peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101895-8 Cry2Ae | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), Fall armyworm (Spodoptera frugiperda))
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-101874-2 Ubiquitin gene 3 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-104517-2 Actin 2 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-14990-5 Vegetative insecticidal protein 3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-104662-3 PCSV Promoter | Peanut chlorotic streak virus (PCSV, PClSV)
    Promoter
  • BCH-GENE-SCBD-104664-2 TEV 5' Untranslated Region | Tobacco etch virus (TEV)
    Leader
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-100728-3 Dicamba monooxygenase gene | Stenotrophomonas maltophilia (S. maltophilia, Stenotrophomonas)
    Protein coding sequence | Resistance to herbicides
  • BCH-GENE-SCBD-105600-1 E6 gene terminator | Gossypium barbadense (Sea-island cotton, Egyptian cotton, GOSBA)
    Terminator
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)
    Leader
DNA insert from GHB811 (BCS-GH811-4) vector pTSIH09
The GHB811 parental genome contains two gene cassettes: Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (hppd) and Zea mays 5-enolpyruvylshikimate-3-phosphate synthase (2mepsps).

The hppd coding sequence is under control of a Cassava vein mosaic virus promoter and Arabidopsis thaliana histone H4 terminator. A synthetic optimized (N-terminal) transit peptide was included to target the translated HPPD protein to the chloroplast. High levels of expression are expected due to the nature of the viral promoter. The 2mepsps coding sequence is under control of an A. thaliana histone H4 promoter and 3'untranslated region. An A. thaliana histone H3 intron was included to enhance the expression of 2mepsps. A synthetic optimized (N-terminal) transit peptide is also present to direct the protein to the chloroplasts.

Note:
Molecular characterization of the parental event indicated that a single copy of the T-DNA was inserted into the genome without the integration of vector backbone sequences.

DNA insert from T304-40 (BCS-GHØØ4-7) vector pTDL008
The T304-40 parental genome contains two gene cassettes: Bacillus thuringiensis crystal protein cry1Ab and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

The cry1Ab coding sequence is under control of Subterranean clover stunt virus genome segment 7 (Ps7s7) promoter and Flaveria bidentis NADP-malic enzyme 1 terminator. The 5' leader sequence from Oryza sativa GE1 gene was also included in the cassette to enhance the expression of cry1Ab. The bar coding sequence is under control of a Cauloflower mosaic virus 35S promoter and Agrobacterium tumefaciens nopaline synthase terminator. Expression levels from both gene cassettes are expect to occur at high levels to the viral promoters and the leader sequence (as in the case for cry1Ab).

Note:
The T-DNA insertion from the parental genome is truncated. In addition, the parental genome contains the following insertions: a partial NADP-malic enzyme 1 terminator, a partial copy of cry1Ab, with a truncated Ps7s7 promoter (in a tail-to-tail orientation) and partial copy of the bar cassette in which the nopaline synthase is truncated.

DNA insert from GHB119 (BCS-GHØØ5-8) vector pTEM12
The modified cotton contains two gene cassettes: Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) and Bacillus thuringiensis cry2Ae.

The bar coding sequence is under control of a Cassava vein mosaic virus promoter and an Agrobacterium tumefaciens nopaline synthase terminator. The cry2Ae coding sequence is under control of a Cauliflower mosaic virus 35S promoter and terminator. 

Note:
  • Due to the constitutive nature of the promoters, high levels of transcription from the bar and cry2Ae gene cassettes are expected.
  • Southern Blot analysis indicated that a single copy of the insert is present in the GHB119 parental line and a configuration that correspond to that in the original vector. No insertion of plasmid backbone sequences were detected, however three ORF were present at the junctions of the integration site but do not have the required regulatory elements for functionality. 

DNA insert from COT102 (SYN-IR1Ø2-7) vector pCOT-1
The DNA insert from COT102 contains two gene cassettes: Escherichia coli hygromycin B phosphotransferase (hpt) and Bacillus thuringiensis vegetative insecticidal protein 3A (vip3Aa).

Transcription of hpt is under control of the Arabidopsis thaliana ubiquitin 3 promoter and the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The gene cassette is present in the counterclockwise orientation. The expression of Bacillus thuringiensis vip3Aa is under transcriptional control of the A. thaliana actin 2 promoter and the nos terminator.

Note:
  • The coding sequence of the vip3Aa was optimized for expression in plant cells.
  • Transcription is expected to occur at elevated levels due to the constitutive nature of the promoters.
  • Southern blot analysis indicated the incorporation of a single copy of the transgene cassettes without the integration of the vector backbone sequences.
  • Western blot analysis confirmed the expression of the proteins.

DNA insert from MON87701 (MON-887Ø1-3) vector PV-GHHT6997
The modified cotton contains two gene cassettes: Stenotrophomonas maltophilia dicamba monooxygenase (dmo) and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

The dmo coding sequence is under control of a Peanut chlorotic streak virus promoter and Gossypium barbadense E6 terminator. A Tobacco etch virus 5' untranslated region was added between the promoter and dmo coding sequence to enhance transcription of dmo.

The bar coding sequence is under control of a Cauliflower mosaic virus 35S enhanced promoter and an Agrobacterium tumefaciens nopaline synthases terminator. A Petunia hybrida heat shock protein 70 leader was added between the viral promoter and the bar coding sequence to enhance expression of bar.

Note:
Southern Blot and sequence analysis indicated that the transformation cassette was inserted into the MON88701 genome at a single intact locus containing all the genetic elements without the integration of vector backbone sequences.

For more information, kindly refer to the parental records.
EN
LMO characteristics
EN
  • Feed
  • Fiber/textile
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1