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Living Modified Organism
(LMO)
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Poplar with an altered wood composition
EN
Poplar SCO
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- Organization: VIB - Flanders Interuniversity Institute for Biotechnology () | BCH-CON-SCBD-42293-1Organization:VIB - Flanders Interuniversity Institute for Biotechnology ()Rijvisschestraat 120 9052 Zwijnaarde, Belgium Tel.: +09 244 66 11,
, BelgiumPhone:Fax: +09 244 66 10,Email: vib@vib.be,Website: www.vib.be,
The genetically modified poplars (Populus canescens) have an altered wood lignin composition resulting from the expression of Arabidopsis thaliana courmain synthase and feruloyl CoA ortho-hydroxylase 1. These enzymes promote the synthesis of scopoletin, which then is added to lignin as a monomer. Overall, the amount of lignin in the cell wall has not changed, but the presence of the scopoletin monomer results in lignin that is less recalcitrant during processing. In research, it was observed that saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was between 30% and 40% higher than for the wild-type. In addition, the modified poplars express Escherichia coli neomycin phosphotransferase II, which permitted the kanamycin or neomycin selection during transformation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-101269-5 Organism Populus canescens (Gray poplar, Grey poplar, Hybrid poplar)Trees
Poplar breeding line 717-1-B4
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pK7m24GW,3
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103358-4 AttB λ attachment site | Escherichia coli (ECOLX)Excision-integration site
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-277173-1 Feruloyl CoA ortho-hydroxylase 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in quality and/or metabolite content (Lignin)
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BCH-GENE-SCBD-277176-1 Coumarin synthase | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in quality and/or metabolite content (Lignin)
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BCH-GENE-SCBD-277139-1 Cellulose synthase A8-B promoter | Populus trichocarpa (Western balsam poplar, Black cottonwood, California poplar, POPTR)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-277212-1 2A-like self-processing peptide | Thosea asigna virus (THAV)Spacer
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-111595-1 Neomycin phosphotransferase II promoter | Escherichia coli (ECOLX)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
The modified poplar expresses an Arabidopsis thaliana courmain synthase (cosy) - feruloyl CoA ortho-hydroxylase 1 (f6'h1) polycistronic cassette and an Eschercihia coli neomycin phosphotransferase II (nptII) cassette.
Expression of the polycistronic cassette is under control of Populus trichocarpa cellulose synthase A8-B promoter and Cauliflower mosaic virus 35S terminator. The nascent transcript contains both COSY and F6'H1 sequences separated by a Thosea asigna virus 2A-like self-processing peptide coding sequence. Following translation, ribosome skipping occurs in the 2A-like peptide. Thus, peptide bonds fail to form and the COSY and F6'H1 proteins are then separated. This 2A-like peptide system allows for the expression of two proteins from a singular mRNA transcript. Expression from this cassette is restricted to phloem fibers, xylem fibers and xylem ray parenchyma cells due to the nature of the promoter.
Expression of nptII is under control of an Agrobacterium tumefaciens nopaline synthase promoter and terminator.
Expression of the polycistronic cassette is under control of Populus trichocarpa cellulose synthase A8-B promoter and Cauliflower mosaic virus 35S terminator. The nascent transcript contains both COSY and F6'H1 sequences separated by a Thosea asigna virus 2A-like self-processing peptide coding sequence. Following translation, ribosome skipping occurs in the 2A-like peptide. Thus, peptide bonds fail to form and the COSY and F6'H1 proteins are then separated. This 2A-like peptide system allows for the expression of two proteins from a singular mRNA transcript. Expression from this cassette is restricted to phloem fibers, xylem fibers and xylem ray parenchyma cells due to the nature of the promoter.
Expression of nptII is under control of an Agrobacterium tumefaciens nopaline synthase promoter and terminator.
EN
- Other (Pulp and paper)
EN
The lignin engineering did not result in alterations to plant growth.
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
