The cotton (Gossypium hirsutum) was produced through cross‑breeding of modified parental varieties BCS-GH811-4, BCS-GHØØ4-7, BCS-GHØØ5-8, and SYN-IR1Ø2-7.

Cotton BCS-GH811-4 was modified for tolerance to glyphosate and isoxaflutole herbicides. To achieve isoxaflutole tolerance, the cotton expresses Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), which contains a point mutation that confers reduced binding affinity to HPPD inhibitors. To achieve tolerance to glyphosate, the cotton expresses the Zea mays double mutant 5-enolpyruvylshikimate-3-phosphate synthase, which has a reduced binding affinity to the herbicide.
Cotton BCS-GHØØ4-7 was modified for resistance against lepidopteran insect pests and glufosinate-ammonium tolerance. To achieve resistance against Lepidoptera, the cotton expresses the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki, which produces a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve glufosinate tolerance, the cotton expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase encoding pat gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
Cotton BCS-GHØØ5-8 was modified for lepidopteran insect pests and glufosinate-ammonium tolerance. To achieve resistance against Lepidoptera, the cotton expresses the cry2Ae gene from Bacillus thuringiensis, which produces a crystal delta-endotoxin Cry2Ae with a pore-forming mode of action in the midgut epithelium of feeding larvae. To achieve glufosinate tolerance, the cotton expresses the the bialaphos resistance gene from Streptomyces hygroscopicus to produce phosphinothricin N-acetyltransferase, which inactivates the herbicide by acetylating L-glufosinate ammonium.
Cotton SYN-IR1Ø2-7 was modified for resistance against lepidopteran insect pests such as the cotton bollworm (Helicoverpa zea), tobacco budworm (Heliothis virescens), pink bollworm (Pectinophora gossypiella), fall armyworm (Spodoptera frugiperd), beet armyworm (Spodoptera exigua), soybean looper (Pseudoplusia includens), cabbage looper (Trichoplusia ni), and cotton leaf perforator (Bucculatrix thurberiella). The LM cotton was also modified to express a selectable marker during transformation. To achieve lepidopteran resistance, the cotton expresses Bacillus thuringiensis vegetative insecticidal protein 3Aa (Vip3Aa), which causes gut paralysis and lysis of epithelium cells of feeding larvae. Vip3Aa is a vegetative insecticidal protein that binds to specific midgut receptors distinct from those recognized by Cry proteins and disrupts the integrity of epithelial cell membranes. For the selection marker, the cotton expresses Escherichia coli hygromycin-B phosphotransferase is also expressed.