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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant eucalyptus
EN
1521K059
Yes
FGN-Ø6K59-4
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Organization:Suzano S.A. ()Escritório São Paulo Avenida Brigadeiro Faria Lima, 1355São Paulo, São Paulo
, BrazilPhone: +55 800 022 1727, +55 11 3503 9000,Fax:Email: suzanoresponde@suzano.com.br,Website: https://www.suzano.com.br/, -
,Organization:FuturaGene Brasil Tecnologia Ltda ()Private sector (business and industry)
, BrazilPhone: + 55 15 3511 8032,Fax:Email: info@futuragene.com,Website:
The eucalyptus (Eucalyptus urophylla) was modified for resistance to insect pests of the order Lepidoptera, particularly of the family Geometridae, such as Thyrinteina arnobia, which is considered on the principle pests of eucalyptus trees. To accomplish this, the eucalyptus tree expresses three Bacillus thuringiensis crystal proteins Cry1Ab, Cry1Bb and Cry2Aa, which have a pore forming mode-of-action in the epithelial lining of feeding larvae. In addition to the insect resistance gene cassettes, a Escherichia coli neomycin phosphotransferase II cassette was also included, which functioned as a kanamycin selectable marker during transformation of the parental lines.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-272517-3 Organism Eucalyptus urophylla (Timor mountain gum, Timor white gum, Eucalyptus, EUCUR)Trees
Clone FGN-K
EN
pBI121
EN
- Agrobacterium-mediated DNA transfer
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0.253 kb
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1.869 kb
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0.067 kb
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0.371 kb
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0.401 kb
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0.795 kb
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0.140 kb
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0.430 kb
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0.253 kb
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1.902 kb
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0.940 kb
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0.371 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-272524-1 Ribulose-1,5-bisphosphate carboxylase/oxygenase small chain terminator | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Terminator
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BCH-GENE-SCBD-272521-2 Cry1Bb | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-272523-1 Ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-115762-1 Leader sequence | Tobacco etch virus (TEV)Leader sequence
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BCH-GENE-SCBD-272522-1 Cry2Aa | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Diptera (flies), Hessian fly (Mayetiola destructor), Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-272525-1 Elongation factor EF-1 alpha | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Intron
The modified eucalyptus contains four gene cassettes: Bacillus thuringiensis crystal protein Cry1Ab, B. thuringiensis crystal protein Cry1Bb, Escherichia coli neomycin phosphotransferase II (nptII) and B. thuringiensis crystal protein Cry2Aa.
Transcription of cry1Ab is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. A Tobacco mosaic virus omega 5' untranslated leader was also included to enhance translation of the Cry1Ab protein. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry1Bb is under control of a Eucalyptus grandis ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter and terminator. The promoter contains the 5' untranslated region and intron to improve transcription. The promoter may be most active in green tissues.
Transcription of nptII is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was also added to enhance translation of nptII. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry2Aa is under control of a CaMV 35S promoter and a nos terminator. An E. grandis elongation factor EF-1 alpha intron was included to enhance the expression of Cry2Aa. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Note:
Transcription of cry1Ab is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. A Tobacco mosaic virus omega 5' untranslated leader was also included to enhance translation of the Cry1Ab protein. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry1Bb is under control of a Eucalyptus grandis ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter and terminator. The promoter contains the 5' untranslated region and intron to improve transcription. The promoter may be most active in green tissues.
Transcription of nptII is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was also added to enhance translation of nptII. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry2Aa is under control of a CaMV 35S promoter and a nos terminator. An E. grandis elongation factor EF-1 alpha intron was included to enhance the expression of Cry2Aa. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Note:
- Next generation sequencing analyses indicated that eucalyptus genome contains a singular T-DNA insertion in chromosome 3 (located at approximately 66 Mbp) without integration of vector backbone sequences. The analyses also indicated that the four cassettes were present as expected (without rearrangements).
- ELISA analyses confirmed expression of Cry1Ab, Cry1Bb, Cry2Aa and NPTII in leaves (young and mature), stems, roots and pollen.
EN
- Timber
- Other (Paper pulp)
EN
EN
- Parecer Técnico 8393_2023.pdf [ Portuguese ]
- GenBank - Eucalyptus urophylla transgenic isolate 1521K059 genomic sequence [ English ]
- Genetically engineered eucalyptus expressing pesticidal proteins from Bacillus thuringiensis for insect resistance: a risk assessment evaluation perspective.pdf [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Recipient Organism or Parental Organisms | 3 | |
