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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, high yield eucalyptus
EN
H421 × 955S019
Yes
FGN-Ø1A21-5 × FGN-Ø3S19-5
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,Organization:FuturaGene Brasil Tecnologia Ltda ()Private sector (business and industry)
, BrazilPhone: + 55 15 3511 8032,Fax:Email: info@futuragene.com,Website: -
Organization:Suzano S.A. ()Escritório São Paulo Avenida Brigadeiro Faria Lima, 1355São Paulo, São Paulo
, BrazilPhone: +55 800 022 1727, +55 11 3503 9000,Fax:Email: suzanoresponde@suzano.com.br,Website: https://www.suzano.com.br/,
The hybrid eucalyptus was modified for tolerance to herbicides and greater biomass. For tolerance to glyphosate, the modified eucalyptus expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which does not bind the herbicide with high affinity and thus allows for the continued functioning of the shikimate pathway and biosynthesis of aromatic amino acids and compounds. For greater yield and increased growth rate, the eucalyptus overexpresses Arabidopsis thaliana endo-β‐1,4‐d-glucanase, which remodels the xyloglucan-cellulose matrix of the cell wall during development to promote cell expansion and growth. In addition to these cassettes, the modified eucalyptus contains two Escherichia coli neomycin phosphotransferase II cassettes, which functioned as kanamycin selectable markers during transformation of the parental lines.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-272517-3 Organism Eucalyptus urophylla (Timor mountain gum, Timor white gum, Eucalyptus, EUCUR)Trees
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BCH-ORGA-SCBD-272520-2 Organism Eucalyptus grandis × Eucalyptus urophylla (Hybrid eucalyptus)Trees
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BCH-LMO-SCBD-109049-3 Living Modified Organism FGN-Ø1A21-5 - Eucalyptus modified for increased growth and yieldFuturaGene Brasil Tecnologia Ltda | Changes in physiology and/or production (Yield), Changes in quality and/or metabolite content (Cellulose), Resistance to antibiotics (Kanamycin, Neomycin), Selectable marker genes and reporter genes
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BCH-LMO-SCBD-272464-2 Living Modified Organism FGN-Ø3S19-5 - Herbicide-tolerant eucalyptusFuturaGene Brasil Tecnologia Ltda, Suzano S.A. | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to herbicides (Glyphosate), Selectable marker genes and reporter genes
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pBI121
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-109045-1 Strawberry vein banding virus promoter | Strawberry vein banding virus (SVBV)Promoter
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BCH-GENE-SCBD-109046-1 Endo‐1,4‐β‐d‐glucanase coding sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in physiology and/or production (Yield)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-115762-1 Leader sequence | Tobacco etch virus (TEV)Leader sequence
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
DNA insert from H421 (FGN-Ø1A21-5)
The parental eucalyptus contains two gene cassettes: Arabidopsis thaliana endo-β‐1,4‐d-glucanase (cel1) and Escherichia coli neomycin phosphotransferase II (nptII).
The cel1 sequence is under control of a Strawberry vein banding virus promoter and an Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the viral promoter, which induces strong constitutive transcription.
The nptII sequence is under control of a nopaline synthase promoter and terminator.
Note:
The cel1 sequence is under control of a Strawberry vein banding virus promoter and an Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the viral promoter, which induces strong constitutive transcription.
The nptII sequence is under control of a nopaline synthase promoter and terminator.
Note:
- Molecular analysis (Southern blot, sequencing and PCR) showed that a single T-DNA insertion occurred into the eucalyptus genome.
- Sequencing of event H421 confirmed that constructs present in the vector were preserved in the transformation process (without rearrangement of the gene cassettes or truncation of the inserted T-DNA sequence).
DNA insert from 955S019 (FGN-Ø3S19-5)
The parental eucalyptus contains three gene cassettes: two Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) cassettes and one Escherichia coli neomycin phosphotransferase II (nptII) cassette.
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
- Molecular characterization indicated that a single T-DNA insertion occurred in the parental eucalyptus genome (in chromosome 3) without inclusion of vector backbone sequences. The sequences are present in the same arrangement as the transformation vector (i.e., without rearrangement). Protein expression was confirmed with ELISA.
For more information, kindly refer to the parental LMO records.
EN
- Timber
- Other (Paper pulp)
EN
EN
